ABSTRACT
Zinc supplementation can reduce the mean duration,the recurrence rate and mortality of diarrhea.Nonetheless,it has no effect on stool frequency or stool output.Zinc supplementation results in different effort for the difference of the duration of diarrhea,pathogens,zinc type and doses of zinc,and shows no effect in infants younger than 6 months.Using iron as a co-intervention can reduce the effect of zinc,but vitamin A or ORS have a synergistic effect with the meeting.Average baseline zinc levels did not contribute to variations in the effect size.Zinc supplements are unlikely to improve compliance with the treatment for diarrheal disease.Longer course,zinc alone,malnutrition,diarrhea,breast-feeding children are prone to vomiting after oral administration of zinc.
ABSTRACT
Objective To explore the distribution pattern of G protein-coupled receptor family C, group 6, subtype A (GPRC6A) mRNA in adult mice. Methods The distribution of GPRC6A mRNA in paraffin embedded adult mouse tissues was determined by highly sensitive nonradioactive cRNA probe in situ hybridization (ISH). We compared ISH with and without addition of tyramide signal amplification (TSA). GPRC6A wild-type and littermate GPRC6A null mice tissue sections were investigated by ISH. Results TSA greatly increased the sensitivity of ISH to detect GPRC6A mRNA in wild type mouse tissues. There was no detection of GPRC6A mRNA in GPRC6A gene specific knockout tissue in paraffin embedded tissue section. The mRNA of GPRC6A was detectable in the digestive gland or accessory digestive gland including salivary gland and pancreas, as well as in the tissues including kidney, testis, brain, muscle, and fat. Conclusion The mRNA distribution pattern of GPRC6A gene is compatible with the phenotype of GPRC6A knockout mice.