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Objective To evaluate the feasibility of contrast material on image quality of coronary CT angiography (CCTA)by using double injection technology and iterative reconstruction.Methods 120 patients with suspected coronary heart disease who underwent CCTA were randomly divided into two groups.Then,60 patients with 30 kg/m2>BMI≥25 kg/m2 were averagely divided into A1 and B1 groups,and other 60 ones with BMI0.05).There was no significant difference in mean image quality scores between group A2 and B2 (P>0.05), however,there were significant differences in the SI,SD of values and SNR,CNR of ascending aorta (P0.05).There was significant difference in effective dose between subgroups (P<0.05).Conclusion The method with a combination of iterative reconstruction and a contrast material of 245 mg I/mL using double inj ection technology can improve the contrast enhancement without impairing image quality.
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We studied the function and mechanism of miR-24 in regulating beta-like globin gene expression. We first detected the expression of miR-24 during erythroid differentiation and also detected the globin gene expression in miR-24 overexpressing K562 cells through q-PCR. Dual-luciferase reporter assay and Western blotting were used to identify target genes of miR-24. "Rescue experiment" was further used to investigate the regulation of miR-24 on globin gene expression whether depending on targeting Sp1 or not. We found that miR-24 increased during hemin-induced K562 cells and EPO-induced HPCs (hematopoietic progenitor cells) erythroid differentiation. Overexpression of miR-24 in K562 cells promoted the epsilon- and gamma-globin gene expression during hemin-induced erythroid differentiation through targeting the negative globin regulator Sp1. These results suggested that miR-24 can improve the expression of beta-like globin gene through targeting Sp1.
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Humans , Cell Differentiation , Gene Expression Regulation , Hematopoietic Stem Cells , Metabolism , K562 Cells , MicroRNAs , Genetics , Sp1 Transcription Factor , Genetics , epsilon-Globins , Genetics , gamma-Globins , GeneticsABSTRACT
Objective To investigate the relationship between apolipoprotein E (ApoE) ε4,ε2 alleles and intrauterine growth.Methods ApoE genotypes of 1418 people born in Peking Union Medical College Hospital were analyzed,allele frequencies were calculated and their parameters at birth were collected.To compare ApoE ε4,ε2 alleles with parameters at birth through single factor analysis and Logistic regression analysis.Results The alleLic frequencies of e2,83 and 84 were 8.11%,83.39% and 8.50% in the group.The results of single factor analysis showed that there was significant difference between the distribution of ponderal index (PI) in the ApoE ε2 allele group(χ2=4.87 ,P=0.027).While there was no significant difference between the distribution of head circumference at birth,placental weight and gestational age in the ApoE ε2 allele group.ApoE ε2 allele showed negative correlation with small PI in the logistic regression analysis(χ2=5.077 ,P=0.024),after adjusted for gender,age,head circumference at birth,placental weight,gestational age,parity and maternal age at delivery.No association between ApoE ε4 allele and parameters at birth was found.Conclusions ApoE ε2 allele may have protective effect on PI.No association was found between ApoE ε4 allele and intrauterine growth.
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BACKGROUND:The polylactic acid/poly plycolic acid (PLGA) exhibits excellent biomechanical property with poor cell adhesion. However,acellular cartilaginous matrix had good cell adhesion and hydrophilia,which can mediate signal conduction and interaction among cells,yet the biomechanical property,is poor. PLGA-acellular cartilaginous matrix scaffold is a remedy for their shortcomings,which can be a newly scaffold materials. OBJECTIVE:To observe the ability of PLGA,acellular cartilaginous matrix,PLGA-acellular cartilaginous matrix scaffold in supporting the growth of porcine chondrocytes for cartilage tissue engineering. DESIGN,TIME AND SETTING:The comparative observation experiment was performed at the Laboratory of Shanxi Medical University from March to September 2008. MATERIALS:The average aperture of PLGA is from 100 -200 ?m,with porosity rate of 94%. The average aperture of the acellular matrix scaffold ranged 70-100 ?m,with porosity rate of 85%. The average aperture of PLGA-acellular matrix aperture is 100-300 ?m,and the porosity rate is approximate 90%. METHODS:The experiment was divided into three groups according to the scaffolds:PLGA,acellular cartilaginous matrix,and PLGA-acellular cartilaginous matrix group. The free chondrocytes isolated from porcine knee articular were seeded onto 3 kinds of scaffolds after cartilage amplification,followed by 8 weeks culture in vitro. MAIN OUTCOME MEASURES:The expression of type Ⅱ collagen was detected by immunohistochemistry. The contents of mRNA of type Ⅱ collagen were determined by RT-PCR. Meantime,the content of DNA was measured by Hoechst 33258 method. RESULTS:Chondrocytes grows vigorous on the PLGA-acellular cartilaginous matrix scaffold,which can maintain phaenotype after 8 weeks of culture,and the secretion of type Ⅱ collagen was superior to other 2 groups. Meanwhile,the content of DNA and mRNA of type Ⅱ collagen of PLGA-acellularcartilaginous matrix groups were greatest,followed by acellular cartilaginous matrix group,and the content of PLGA was smallest. The one-factor analysis of variance showed the difference had significant (P
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OBJECTIVE:To determine the content of ?-schizandrin,one of the effective ingredients,in Shuangjia Wuling capsules METHODS:The RP-HPLC method was performed with YWG C18 column(4 6mm?250mm) The mobile phase consisted of methanol-water(72∶28) The detecting wavelength was 254nm RESULTS:The calibration curve for ?-schizandrin was linear in the range of 0 0 207～0 4 130mg/ml(r=0 9 999) The average recovery was 97 68% with RSD=1 85% CONCLUSION:The method is simple,rapid and reliable for quality control of the capsules
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Objective To identify and clone rat aging-related genes to provide clues for human aging mechanism. Methods Improved different display reverse transcript-PCR method was applied to identify differentially expressed genes in cortex tissues of 4-month and 24-month old BALB/c mouse. Results Forty-two cDNA fragments with differential expression were identified, and 21 with increase and 21 with decrease of expression in old mice. Among them, 17 represented genes with known protein function, 12 represented known gene sequences but their protein function was unknown, and the other 13 probably belonged to new cDNAs. Among the genes with known protein function, 2 genes were associated with oxidative stress, 3 with energy production, and 4 with protein metabolism, respectively. Additionally, gene expression alterations were also found in those related to cell apoptosis, neurodegenerative disorder, and growth and development regulation. Conclusions Rat aging might be related with the alteration of oxidative stress status, energy production and protein metabolism.