ABSTRACT
OBJECTIVE To investigate dopamine level changes in the Nucleus Accumbens (NAc) of the limbic system by salicylate and the role of dopamine in the mechanisms of tinnitus.METHODS We recorded the change of dopamine before and after sodium salicylate injection in rats by microdialysis combined with electrochemical methodologies in the nucleus accumbens (NAc).RESULTS Salicylate produced significant increase in dopamine level in the NAc, to a maximum of (321±97)% of baseline and then remain stable. In contrast, saline only produced slight increase.CONCLUSION The increase of dopamine in the NAc may be related to the generation of tinnitus. The data in vivo provide direct evidence of the important role that the dopaminergic system plays in the generation of tinnitus.
ABSTRACT
Objective To investigate the postoperative self-care ability and its influence factors in patients with esophageal cancer. Methods The self-made basic status questionnaire, exercise of self-care agency scale and social support rating scale were used to survey 106 cases with esophageal cancer from Jan. 2012 to Dec. 2014. The risk factors of self-care ability were analyzed. Results 101 effective questionnaire were got and the effective rate was 95.28%. The total score of self-care ability was (108.6± 21.5), 45 cases (44.55%) were at higher level and 56 cases (55.45%) were at moderate level. Univariate analysis showed educational level, chronic health disorders and health perception were associated with the total score of postoperative self-care ability (All P<0.01). The total score of social support were (43.5±6.5) and positively correlated with self-care ability (r=0.325,P<0.01). The multiple linear regression analysis revealed that education level, health perception and social support were important risk factors of self-care ability (t=2.926,-3.654, 2.301, All P<0.05 or 0.01). Conclusions The level of postoperative self-care ability in patients with esophageal cancer is not high. For the patients with low level of education, health perception and social support, nurse should enhance the health education.
ABSTRACT
Cerebellum connects with each part of the auditory pathway directly or indirectly, forming the anatomical basis for a cerebellar role in auditory process. Functional imaging studies and clinical observations provide more abundant support for this view. Tinnitus is one of the most common clinical manifestations when the auditory system is impaired and remains unsolved. Basic science researches in neurotransmitter chemistry and molecular biochemistry, together with functional imaging studies, indicate that cerebellum might contribute to the pathophysiology of tinnitus. In this review we summarize the current understanding of cerebellar role in auditory process and tinnitus.
Subject(s)
Humans , Auditory Pathways , Cerebellum , TinnitusABSTRACT
A case of large artificial teeth in hypopharynx and upper esophagus was reported. Physical signs of laryngeal obstruction were detected. CT showed foreign body in right pyriform sinus. Acute tracheotomy was performed before removal of the large foreign body with the help of esophagoscope and mouth-gag.
Subject(s)
Humans , Male , Middle Aged , Esophagus , Foreign Bodies , HypopharynxABSTRACT
OBJECTIVE To study the expression of CSE1L gene sifted from the whole genome expression profiling in the aRNA from homogenous human nasopharyngeal carcinoma (NPC) and non-NPC biopsy tissue samples using semi-quantitative RT-PCR (sqRT-PCR). METHODS CSE1L gene was sifted from the whole genome expression profiling from homogenous NPC and non-NPC biopsy tissue samples. RNA later RNA Stabilization Reagent was used to preserve the tissue samples harvested from the nasopharynx of NPC and non-NPC patients. The samples were microdissected to get the homogenous tissue cells and then the total RNA was isolated from them. The antisense RNA (aRNA) was amplified from the total RNA by “in vitro transcription” (IVT). CSE1L gene expression in the homogenous tumor cells of NPC and the pure epithelial pillar cells of normal nasopharyngeal tissue was investigated by sqRT-PCR. RESULTS The high quality total RNA could be harvested from the microdissected homogenous tissue cells, and then the sufficient aRNA was amplified from it. The expression of CSE1L gene was identified using these aRNA by sqRT-PCR. There were significantdifference between the average expression value of CSE1L in NPC tissue (1.740?1.105) and in non-NPC tissue (0.618?0.183; df=30, t=3.159, P=0.004). The expression of CSE1L gene in the whole expression profiling were 1.056?0.296 in NPC tissue and 0.465?0.835 in non-NPC tissue respectively (df=16, t=4.317, P=0.001) . CONCLUSION The whole genome expression profiling with sqRT-PCR could be used to sift the marker genes from biopsy tissue samples. CSE1L may be as a candidate oncogene in NPC.