ABSTRACT
To investigate the temporal variation of cell-mediated immunity in injured tissue of maxillofacial firearm trauma. Cells expressing messenger RNA(mRNA) for IL-2 were demonstrated by dot blotting and in situ hybrization in injured tissue of maxillofacial trauma inflicted by bullet, in conjunction with immunohistochemistry for IL-2 in normal and postinjury conditions. It was found that, apart from a transient eleratim in level within 24h post injury, both IL-2 production and IL-2 mRNA expression were consistently suppressed in cells at the site of the wound compared with the normal. The changes in the tissues of indirect injury by the projectile were significant. These results indicate that the cell-mediated immunity was suppressed in maxillofacial firearm trauma and the principal cellular abnormalities that resulted in altered T cell activation and IL-2 production postinjury was downregulation of mRNA before IL-2 gene transcription. It also suggested that the indirect injury by projectile contribute mainly to the depression of cell-mediated immunity.
ABSTRACT
To investigate the temporal variation of cell mediated immunity in injured tissue of maxillofacial firearm trauma. Cells expressing messenger RNA(mRNA) for IL 2 were demonstrated by dot blotting and in situ hybrization in injured tissue of maxillofacial trauma inflicted by bullet, in conjunction with immunohistochemistry for IL 2 in normal and postinjury conditions. It was found that, apart from a transient eleratim in level within 24h post injury, both IL 2 production and IL 2 mRNA expression were consistently suppressed in cells at the site of the wound compared with the normal. The changes in the tissues of indirect injury by the projectile were significant. These results indicate that the cell-mediated immunity was suppressed in maxillofacial firearm trauma and the principal cellular abnormalities that resulted in altered T cell activation and IL 2 production postinjury was downregulation of mRNA before IL 2 gene transcription. It also suggested that the indirect injury by projectile contribute mainly to the depression of cell mediated immunity.