Quantification of Stigmasterol in Successive Extracts of Clerodendrum Serratum, Poly Herbal Formulation By Hptlc Method And In-vitro Anti - Oxidant Activity Studies.

The aim of the present study is to carry out the quantification of Clerodendrum serratum containing poly herbal formulations by HPTLC and to study the invitro anti-oxidant activity of nitric oxide scavenging activity of the roots of Clerodendrum serratum.The rhizome part of Clerodendrum serratum has been selected, dried, powdered and extracted with petroleum ether, chloroform, ethyl acetate and methanol successively by continuous hot percolation method using soxhlet apparatus. Preliminary phytochemical screening of the successive extracts showed the presence of chemical constituents like triterpenoids, flavonoids, alkaloids, tannins, glycosides and sesquiterpenes. The Rf value of stigmasterol was found to be 0.80 ± 0.02.The developed methods were validated as per ICH guidelines. The LOD and LOQ was found to be 100 and 300 ng/spot respectively for HPTLC. The anti-oxidant activity was highest in petroleum ether extract when compared to the other extracts by using ascorbic acid as control. This shows that the polyherbal formulation containing stigmasterol has medicinal value and that it may be utillised for its anti-oxidant properties.

Quantitative Estimation of Lupeol in Polyherbal Formulation And Successive Extracts of Crataeva Nurvala and their In-Vitro Anti-Inflammatory Activity Study.

The aim of the present study is to carry out the quantitative estimation of lupeol in poly herbal formulations by HPTLC and to study the invitro antiinflammatory activity study of Crataeva nurvala bark. The bark of Crataeva nurvala was selected, dried, powdered and extracted with petroleum ether, diethyl amine and methanol successively by continuous hot percolation method using soxhlet apparatus. Preliminary phytochemical screening of the successive extracts showed the presence of chemical constituents like triterpenoids, flavonoids, alkaloids, tannins, glycosides and sesquiterpenes. The Rf value of lupeol was found to be 0.49 ± 0.03.The developed methods were validated as per ICH guidelines. The LOD and LOQ was found to be 50 and 300ng/spot respectively for HPTLC. The anti-inflammatory activity was highest in petroleum ether extract when compared to other extracts when evaluated by using ibuprofen as the control. This shows that the polyherbal formulation and medicinal plants containing lupeol as the active constituent has medicinal value and that they may be utillised for their anti-inflammatory properties.