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Background: Autoimmune haemolytic anaemia (AIHA) is relatively uncommon condition with grave consequences, if not diagnosed and treated early. The literature on the clinical outcome and response to treatment is relatively scarce. Aim was to study the clinic-pathological profile and the treatment outcomes in patients with AIHA.Methods: Around 25 patients with AIHA attending a tertiary care hospital over a period of one year were included in the study. The patients were divided based on severity of anaemia and etiology. All the patients data were analysed for the demographic data, clinico -pathological findings and the response to treatment. All the patients data were analysed using SPSS software (version 22).Results: Out of 25 patients, 76% were females and 24% were males. Based on severity of anaemia, 60%, 28% and 8% had severe, moderate and mild anaemia. Around 48% of the patients had thrombocytopenia along with anaemia. 8 (32%) and 17 (68%) patients have primary and secondary AIHAs respectively. In our study the commonest cause for the secondary AIHA was Systemic Lupus Erythematosus (SLE) followed by haematological malignancy, primary Sjogrens, Anti-phospholipid antibody (APLA) syndrome, carcinoma colon and Wilsons disease. Hepatosplenomegaly and lymphadenopathy were present in 36% and 4% respectively. Out of 22 (88%) patients on corticosteroid therapy, 15 (60%) patients responded to corticosteroids alone and 6(24%) patients required corticosteroid plus immunosuppressive therapy.Conclusions: AIHA has to be ruled out in all anaemia patients with indirect hyperbilirubinemia and abnormal peripheral smear. Secondary AIHA is more common than primary. Corticosteroids and immunosuppressive agents are the mainstay of treatment of AIHA.
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Aquaculture is the farming of economically important aquatic animals and plants under controlled conditions. The analysis of enzyme profile of Indian major and exotic carps were carried out. The study revealed that there was fluctuation in physicochemical parameters. The enzyme profile in the present study the major carps showed significantly higher concentration than the exotic craps
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Objective: This research investigated on in vitro ffree-radical scavenging property for the alcoholic extract from Pseudarthria viscida Linn. [Leguminosae]. Different antioxidant models of screening were employed
Material and methods: The alcoholic root extract of P. viscida was screened for free radical scavenging potential using 2, 2- azinobis- [3- ethyl-benzothiazoline- 6- sulphonate] [ABTS], 1, 1- diphenyl, 2- picrylhydrazyl [DPPH], superoxide and nitric oxide radicals
Results: Concentration dependent free radical scavenging activity was observed for the extract in which a concentration of 1000 micro g/ ml exhibited maximum scavenging activity against the radical cation, 2,2- azinobis- [3- ethylbenzothiazoline- 6- sulphonate] [99.41%], followed by superoxide radical using Riboflavin- Ethylene diamine tetra-acetic acid [97.60%] and the nitric oxide model [82.50%]. However, only moderate scavenging activity was observed with the 1, 1- diphenyl, 2- picryl hydrazyl radical [59.61%] at the same concentration
Conclusion: All the results of the in vitro antioxidant models of screening revealed the potent free-radical scavenging of the alcoholic root extract of Pseudarthria viscida Linn
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The study aims to examine the Daboia russelli venom neutralization potential of the leaf extract of Ricirioccirpus indicus [Euphorbiaceae] at different dose levels. The acetone extract at two dose level [250 and 500 mg/kg] was studied for haemorrhagic, necrotizing and mast cell degranulation in rats and for its cardiotoxic and neurotoxic effects in isolated frog tissue. Administration of the acetone leaf extract at an i.p. dose level of 500 mg/kg significantly inhibited, in a dose-dependent manner, the Daboia nisselli venom-induced haemorrhagic, necrotizing and mast cell degranulation in rats and the cardiotoxic and neurotoxic effects in isolated frog tissue. Administration of the acetone extract also significantly reduced the Thiobarbituric acid reactive substance levels, enhanced the GST and GSH levels. The observations confirmed that the acetone leaf extract of Ricinoccirpus indicus possesses potent snake venom neutralizing properties
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To evaluate the antidiabetic effects of the alcoholic extract of leaves and stem of I. auamoclit employing a short term study. A short tem study was carried as a preliminary investigation to evaluate the antidiabetic effect of the alcoholic extract of leaves and stem of I. quamoclit and its normoglycemic and antihyperglycemic activity in streptozotocin [STZ[- nicotinamide induced non insulin-dependent diabetes mellitus rats using Glucose Tolerance Test t[GTT]. Graded doses [250 and 500 mg/kg] of the alcoholic root extract suspended in gum acacia were administered to normal and experimental diabetic rats. A significant fall in the blood glucose level of extract-treated animals was seen after 1 hour, indicating its hypoglycemic activity. Continuous blood glucose lowering activity was observed up to 4 hours of administration in normoglycemic and diabetic rats. The results were compared with those following the administration of standard oral hypoglycemic agent, glibenclamide. Administration of the alcoholic extract caused statistically significant decrease in the blood glucose levels of normal and diabetic rats as compared to the normal control and diabetic control groups respectively. Longer duration studies of Ipomoea quamoclit on chronic models may contribute toward the development of a potent antidiabetic drug
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TheplantMarsileaquadrifoliaLinn. [Marsileaceae] has been screened for its hypoglycemic activity in both normal and alloxan-induced diabetic male albino mice. In the present study, the vacuum dried ethanol extract of leaves of Marsilea quadrifolia showed significant hypoglycemic activity. The mice were randomly divided into two main groups. The Group I animals were injected with alloxan mono-hydrate at a dose level of 150mg/kg body weight intraperitoneally. Group II animals were used as normal [nondiabetic]. The diabetic and nondiabetic animals were divided into four sub groups. Mice in first group received 1% CMC, second group received a suspension of the ethanol extract at a dose level of 500 mg/kg and third group was administered with Glibenclamide. The mean glucose levels of mice at various time intervals were observed. The ethanolic extract of M. quadrifolia is endowed with significant anti-hyperglycemic activity thereby justifying its use in the indigenous system of the medicine