ABSTRACT
OBJECTIVE@#To study protein-protein interaction between heterogeneous nuclear ribonucleoprotein H (hnRNP H) and Dengue virus (DENV) proteins.@*METHODS@#DENV proteins were screened against the host hnRNP H protein, in order to identify the host-viral protein-protein interactions in DENV infected THP-1 cells by co-immunoprecipitation. The co-localization of the interacting proteins was further confirmed by immunofluorescence microscopy.@*RESULTS@#The host protein hnRNP H was found to interact with DENV non-structural 1 protein and help the virus to multiply in the cell.@*CONCLUSIONS@#The non-structural 1 glycoprotein is a key modulator of host immune response and is also involved in viral replication. Therefore, disruption of this key interaction between hnRNP H and DENV non-structural 1 could be an important therapeutic strategy for management of DENV infection.
ABSTRACT
Uranium poses both chemical and radiological hazard to the living system. Drinking water from river is one of the major sources of uranium intake. Dissolution of minerals, washout from rain water, rock-water interaction, agricultural run off, and industrial disposals are some of the sources of uranium in river water system. Present study was aimed to determine the uranium in the water Alaknanda and Ganges rivers and its post-monsoon spatial distribution from Nandprayag to Haridwar. River water samples were collected during the post monsoon period in pre-washed polypropylene bottles from the designated locations at both of the rivers. Samples were filtered and analyzed by fluorimetric technique. The measurements showed the concentration of uranium in water of Alaknanda river varied from 3.05 micro g/l to 2.53 micro g/l along the downstream sampled locations with a mean value of 2.75 micro g/l, whereas in water of Ganges river the concentration varied in the range 1.70 micro g/l to 2.00 micro g/l with a mean value of 1.86 micro g/l. The average concentration of uranium was found significantly higher [2.75 micro g/l] in water of Alaknanda river than in Ganges river the average values [1.86 micro g/l] of. However, both the values were far lower than the permissible limits at the sampled locations. The values obtained in present studies were notably higher than that reported elsewhere which seems partly attributable to post monsoon contributing factors
ABSTRACT
Recent results have provided increasing evidence to support involvement of membrane damage in the mechanism of ionizing radiation induced killing of mammalian cells. These findings have stimulated renewed interest in evaluating the damage to membrane as a primary initiator in radiation-induced cell killing especially in apoptotic death. The present study was aimed to gain deeper insight on the molecular mechanisms of radiation-induced damage at membrane level with consequences to apoptotic cell death. Materials and Radiation mediated changes in membrane fluidity in egg yolk lecithin [EYL] liposomal membrane were studied by employing 1,6-diphenyl hexatriene [DPH] fluorescence polarization and subsequent oxidative damage by measurement of malondialdehyde [MDA] by thiobarituric acid reactive species [TBARS] spectrophotometrically. Alterations in membrane permeability and nuclear dimensions in g -irradiated immature mouse thymocytes were investigated by fluorescein diacetate [FDA] and propidium iodide [PI] method, respectively. Results on irradiated liposomes have shown dose dependent lipid peroxidation with concomitant alterations in bilayer fluidity. These parameters were found significantly modified, when liposomes were prepared with a -tocopherol or cholesterol suggesting the contributions of both the radical scavenging and matrix modifying factors in membrane peroxidative damage. In addition, studies on mouse thymocytes labeled with FDA and PI have shown increased permeability of the plasma membrane and decreased nuclear diameter following g -irradiation of thymocytes. Alteration in membrane permeability with the time of post-irradiation incubation was found correlated with the induction of apoptotic death of thymocytes. These results suggest that membrane associated radiation damage were correlated with changes in the nucleus of cells. Moreover, the membrane damage seems a primary trigger in radiation-induced apoptosis which may have implications in cancer radiotherapy