Radioprotective efficacy of Prunus avium fruit in mice brain with reference to in-vitro and in-vivo studies

Radioprotective efficacy of Prunus avium fruit extract [PAE] rich in vitamin A, C, E and anthocyanin was studied against radiation induced biochemical alterations in mice brain. In-vitro assays were performed with PAE for its antioxidant studies; thereafter for in-vivo study, Swiss albino mice were divided into five groups. Group 1 [control] normal mice. Group 2 [PAE treated] PAE supplemented [450 mg/kg. b.wt/day] for 15 days. Group 3 [Irradiated] 5 Gy gamma-radiation treated. Group 4 [PAE + Irradiated] was administered PAE once daily for 15 consecutive days, thereafter exposed to 5 Gy gamma-radiation. Group 5 [Irradiated + PAE] was exposed to 5 Gy gamma-radiation than PAE was administered for 15 consecutive days. Brains were removed at various post treatment intervals for biochemical estimations. The IC50 values of PAE for 2,2-diphenyl-1-picrylhydrazyl [DPPH·] scavenging assays, superoxide radical scavenging assays, inhibition of in vitro lipid peroxidation assays and protein carbonyl formation assay were 413 microg/ml, 7.63 microg/ml, 136.18 microg/ml and 16.94 microg/ml respectively. Furthermore the total phenolic content in PAE was equivalent to 8.38 mg/ml of gallic acid. The rate of ·OH radical scavenging activity of PAE was 0.57 times slower than SCN- derived from nanosecond pulse radiolysis studies. In-vivo study also showed that radiation [5 Gy] induced augmentation in the levels of lipid peroxidation and depletion in glutathione and protein level in mice brain were significantly ameliorated by PAE pre/post treatment. Results suggest that the radioprotective efficacy of PAE may be due its antioxidant property

Hepatoprotective efficacy of Grewia asiatica fruit against oxidative stress in swiss albino mice

The radioprotective effect of Grewia asiatica fruit [GAE] which contains anthocyanin type cyanidin 3- glucoside, vitamin C, A, minerals, carotenes and dietary fibers etc was studied. For study Swiss albino mice were divided into five groups-1. Control [vehicle treated] 2. GAE treated [700 mg / Kg. b.wt / day for fifteen days], 3. Irradiated [5 Gy], 4. GAE + Irradiated and 5. Irradiated + GAE treated. The irradiation of animals resulted in a significant depletion in the DNA and RNA level at all intervals studied viz 1-30 days in comparison to control group. Treatment of mice with GAE before and after irradiation caused a significant elevation in liver DNA and RNA level in comparison to irradiated mice. Photomicrograph of liver histology also showed that pre and post supplementation of GAE provides protection against radiation. Similarly counting of different type hepatocytes also showed that GAE protect the liver against radiation. Thus biochemical and histopathological results proves that GAE has the potential against radiation

Post treatment effect of Grewia asiatica against radiation induced biochemical changes in brain of Swiss albino mice

The aim of the present study was to evaluate the radioprotective effect of Grewia asiatica fruit pulp extract [GAE] on Swiss albino mice exposed to gamma radiation. In the present study radioprotective efficacy of Grewia asiatica [rich in anthocyanin, carotenes, vitamin C, etc.] was studied against radiation induced biochemical alterations in mice cerebrum. For experimental study, healthy Swiss Albino mice were selected from an inbred colony and divided into four groups. Group I [normal] did not receive any treatment. Group II was orally supplemented [GAE] once daily at the dose of 700 mg/kg.b.wt/day for fifteen consecutive days. Group III [control] received distilled water orally equivalent to GAE for fifteen days than exposed to 5 Gy of gamma radiation. Group IV [IR+Drug] was administered orally [GAE] for 15 consecutive days once daily after exposed to single dose of 5Gy of gamma radiation respectively. Mice were sacrificed at different autopsy intervals viz. 1, 3, 7, 15 and 30 days and brain were removed for various biochemical estimations viz. glutathione [GSH], lipid peroxidation [LPO] and protein. GAE post treatment renders protection against various biochemical changes in mice brain. Radiation induced augmentation in the levels of LPO was significantly ameliorated by GAE post-treatment. Radiation-induced depletion in the level of GSH, protein was checked significantly by GAE administration. These results indicate that Grewia asiatica fruit extract [GAE] is able to protect the brain of Swiss albino mice against radiation induced biochemical alterations