Determination of bivalirudin in human blood by HPLC-MS/MS / 中国药学杂志

OBJECTIVE: To establish an HPLC-MS/MS method for the determination of blood-bivalirudin concentration. METHODS: Following a SPE method, the analytes were separated on a C18 column interfaced with a triple-quadrupole tandem mass spectrometer using positive electrospray ionization. RESULTS: The calibration linear rang of blood-bivalirudin concentration was 25-20000 μg · L-1 (r = 0.9969). The between-day and within-day RSD% were less than 6.63%. The recoveries were more than 80%. CONCLUSION: The rapid, sensitive and reliable HPLC-MS/MS method can be applied to monitor blood- bivalirudin profile.