ABSTRACT
Influenza surveillance was conducted in Pune, India in 2003. A total of 573 throat swabs/ nasal swabs (TS/NS) and 190 nasopharyngeal aspirates (NPA) were collected from 763 in- and out-patients who were mostly children aged 0-16 years. TS/NS (507/573) and NPA (42/190) specimens were processed in MDCK cell cultures and identified with the hemagglutination inhibition test (HI). A total of 37 influenza viruses was isolated: twenty-three type A (H3N2) and 14 type B of the Yamagata lineage were isolated from 29 children and 8 adults. Three type A (H3N2) isolates were characterized as being similar to A/Panama/2007/99 like, A/Korea/770/2000 like, and B/Sichuan/379/99 like strains.
Subject(s)
Child , Child, Preschool , Disease Outbreaks , Female , Humans , India/epidemiology , Infant , Infant, Newborn , Influenza A Virus, H3N2 Subtype/isolation & purification , Influenza B virus/isolation & purification , Influenza, Human/epidemiology , Male , Population Surveillance , Time FactorsABSTRACT
A total of 293 patients with influenza like illness were investigated during the course of continuous surveillance on influenza in Pune, India in 2000. The throat/nasal swab specimens collected from these patients were inoculated in MDCK cell culture and influenza types A(H3N2), A(H1N1) and type B strains were isolated. They were identified as similar to the recently prevalent variant strains; A/Sydney/05/97(H3N2), A/New Caledonia/20/99(H1N1) and B/Sichuan/379/99. The latter two were the new variant strains reported for the first time in Pune. It is important to note that A(H1N1) strains were isolated in Pune during 2000 after a gap of 10 yr.
Subject(s)
Animals , Cell Line , Dogs , Genetic Variation , Humans , India/epidemiology , Influenza A Virus, H1N1 Subtype , Influenza A Virus, H3N2 Subtype , Influenza A virus/classification , Influenza B virus/classification , Influenza, Human/epidemiologyABSTRACT
A total of 456 patients with influenza like illness were investigated during the course of continuous surveillance on influenza in Pune, India in 1998. The throat and nasal swab specimens collected from these patients were processed in MDCK cell culture. Influenza type A (H3N2) and type B strains were isolated in MDCK cell culture. They were identified as similar to the recently prevalent globally circulating variant strains; A/Sydney/05/97 (H3N2) and B/Harbin/07/94.