ABSTRACT
OBJECTIVE@#To investigate the enhancement of macrophage chemotaxis in patients with knee osteoarthritis (KOA) and its correlation with the disease severity.@*METHODS@#Eighty patients with KOA admitted from July 2019 to June 2022 were enrolled as the observation group and divided into 29 cases of moderate group, 30 cases of severe group and 21 cases of extremely severe group. At the same time, 30 healthy subjects were included as the control group. The gene expressions of NF-κB, CXC chemokine receptor 7 (CXCR7) and CXC chemokine ligand 12 (CXCL12) in macrophages of each group were analyzed. Visual analogue scale(VAS) was used to evaluate the degree of joint pain. Joint function was evaluated by knee Joint Society Scoring system(KSS). Finally, data analysis was carried out.@*RESULTS@#The expression levels of NF-κB, CXCR7 and CXCL12 in moderate group, severe group and extreme recombination group were higher than those in control group. The VAS, the expression of NF-κB, CXCR7 and CXCL12 in the severe group and the extreme recombination group were higher than those in the moderate group, whereas KSS was lower than that in the moderate group. The VAS, expression levels of NF-κB, CXCR7 and CXCL12 in the extremely severe group were higher than those in the severe group, and KSS was lower than that in the severe group (all P<0.01). The expression levels of NF-κB, CXCR7 and CXCL12 in macrophages were positively correlated with VAS score, but negatively correlated with KSS(all P<0.01). The expression levels of NF-κB, CXCR7 and CXCL12 in macrophages were positively correlated with the severity of disease. After excluding the influence of traditional factors (gender, age and disease duration), multiple linear regression analysis further showed that the expression levels of NF-κB, CXCR7 and CXCL12 were still positively correlated with the severity of disease(all P<0.01).@*CONCLUSION@#The chemotaxis of macrophages in patients with KOA increased with the aggravation of the disease, and was related to the degree of pain and function impairment.
Subject(s)
Humans , Osteoarthritis, Knee/genetics , Chemotaxis/genetics , NF-kappa B/metabolism , Macrophages/metabolism , Receptors, CXCR/metabolism , Patient AcuityABSTRACT
In the present study, two new trinor-guaiane sesquiterpenes, named clavuridins B (1), and A (2), along with three known sesquiterpenes (3-5), were isolated from the Xisha soft coral Clavularia viridis. Their structures and absolute configurations were determined on the basis of spectroscopic analysis, X-ray diffraction analysis with Cu Kα radiation and by comparison with related model compounds. Compounds 1 and 3-5 were evaluated for their cytotoxic activity.
Subject(s)
Animals , Anthozoa , Chemistry , Biological Products , Chemistry , Pharmacology , Magnetic Resonance Spectroscopy , Molecular Structure , Sesquiterpenes, Guaiane , Chemistry , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of inhibited α7 neuronal nicotinic acetylcholine receptor (nAChR) by small interference RNA (siRNA) in SH-SY5Y cells and to explore the connection of these changes with the β-amyloid precursor protein (APP) metabolism and the pathogenesis of Alzheimer's disease (AD).</p><p><b>METHODS</b>The siRNA of α7 nAChR was transfected into SH-SY5Y cells, and the expression of α7 nAChR and two subtypes of β-secretases (BACE1 and BACE2) at mRNA and protein levels was studied by real-time PCR and Western blot, respectively. The variation of Aβ(1-42) content was detected by ELISA.</p><p><b>RESULTS</b>As compared with controls, the expression of α7 nAChR at mRNA and protein levels in the SH-SY5Y cells transfected with the α7 nAChR siRNA were decreased by 84% and 79% (P < 0.01), respectively. The expressions of BACE1 mRNA and protein levels was increased by 527% and 71% (P < 0.01), respectively, while the expression of BACE2 decreased by 58% and 75% (P < 0.01), respectively. The Aβ(1-42) content increased by 208% (P < 0.01).</p><p><b>CONCLUSIONS</b>An inhibited α7 nAChR mRNA induced by siRNA may markedly stimulate the production of Aβ through the mechanism of increased expression of BACE1 and inhibited expression of BACE2, which may be related to the pathogenesis of AD.</p>
Subject(s)
Humans , Amyloid Precursor Protein Secretases , Genetics , Metabolism , Amyloid beta-Peptides , Metabolism , Aspartic Acid Endopeptidases , Genetics , Metabolism , Cell Line, Tumor , Neuroblastoma , Metabolism , Pathology , Peptide Fragments , Metabolism , RNA Interference , RNA, Messenger , Metabolism , RNA, Small Interfering , Genetics , Receptors, Nicotinic , Genetics , Metabolism , Transfection , alpha7 Nicotinic Acetylcholine ReceptorABSTRACT
The argE gene from Escherichia coli coding for N-acety-L-ornithine deacetylase(NAOase), the key enzyme involved in the L-arginine biosynthesis, had been cloned in pET22b and transformed into BL21 (DE3). With 32.5% expression level in the optimal fermentation medium at 37 degrees C, most NAOase was expressed as inclusion bodies. The soluble and active proportion could be slightly increased when expressed at low temperature. The specific activity of soluble NAOase purified by Ni-NTA resin chromatography was 1193.2u/mg. The species and proportions of whole cell proteins varied with induction conditions. The inclusion bodies expressed at 37 degrees C was more pure than 22 degrees C after gradient wash with urea. Inclusion bodies could be partly refolding and reactivated by dilution and dialysis. Low protein concentration and suitable rate of oxidant/reducing agents were important to renaturation. In the optimal conditions 17.78% of Urea-denatured NAOase could be refolding and reactivated by dilution. The purified fusion protein was obtained after wash, solubilization and Ni-NTA resin affinity chromatography purification of inclusion bodies.
Subject(s)
Amidohydrolases , Chemistry , Genetics , Metabolism , Biocatalysis , Electrophoresis, Polyacrylamide Gel , Escherichia coli , Genetics , Escherichia coli Proteins , Genetics , Metabolism , Gene Expression Regulation, Bacterial , Gene Expression Regulation, Enzymologic , Inclusion Bodies , Protein Folding , Recombinant Proteins , Chemistry , Metabolism , Urea , PharmacologyABSTRACT
The kinetics of immobilized cells of Brevibacterium ammoniagenes MA-2 and Brevibacterium flavum MA-3 cells were studied. By means of both a theoretical analysis of diffusion in the gel particles and an experimental determination of apparent kinetic parameters, the intrinsic kinetic parameters of immobilized cells of B. ammoniagenes MA-2 and B. flavum MA-3 cells were obtained.
Subject(s)
Brevibacterium , Metabolism , Physiology , KineticsABSTRACT
Art phonetics' medicine, a new branch of traditional medicine, has not been developed perfectly, especially in the aspects of objective and scientific study. In this paper, the acoustical and anatiomical basis of art phonetics in viewpoint of biomedical engineering is explored, and then our work of quantitative measurement and analysis of art phonetic is introduced. The experiment data show further that quantitative measurement and analysis plays an important role in art phonetic medicine.
Subject(s)
Female , Humans , Male , Acoustics , Biomedical Engineering , Image Processing, Computer-Assisted , Larynx , PhoneticsABSTRACT
In order to change the situations such as students’passivity and low enthusiasm in the traditional experimental teaching of Microbiology and improve students’comprehensive qualities,exploration and attempt on experimental teaching reforms including teaching contents,teaching modes,laboratory management and evaluation system of examination were done.Teaching practices showed that the normalized ex- periment syllabus based on the correct orientation of experimental teaching of Microbiology,the actualization of teaching mode of basic skill training-integrated experiment-designed experiment and the management of opening laboratory were effective measures in improving experimental teaching of Microbiology.
ABSTRACT
The isolated 24 strains-producing hydantoinase & carbamoylase were first identified by Biolog microbial identification system and 16S rDNA sequence analysis.The results suggested that the hydantoinase & carbamoyalse-producing bacteria belonged to Bacillus,Geobacillus,Brevibacillus,Aneurinibacillus,Microbacterium,Pseudomonas,Kurthia and Empedobacter,and so on.Especially,Kurthia and Empedobacter were new hydantoinase & carbamoylase-producing genera.Furthuremore,it was found that D-hydatoinase & carbamoyalse-producing bacteria belonged to Pseudomonas and Agrobacterium,while most of L-hydantoinase & carbamoyalse-producing bacterial belonged to Bacillus,Geobacillus and Microbacterium.The distribution feature of D-hydantoinase & carbamoyalse-producing bacteria and L-hydantoinase & carbamoyalse-producing bacteria showed some genera tendency.This research work will provide the biomaterial of different hydantoinase and carbamoylase and contribute to study the structure and function,molecular evolution of the two enzymes.