ABSTRACT
Objective To investigate the protective role of glycyrrhizin on experimental obstructive nephropathy in rats. Methods The model of obstructive nephropathy was induced by unilateral uretera l ligation in rats and the animals were sacrificed at 12 h and 3,14,28,56 d resp ectively after operation. Specimens were taken from the cortex of kidney. In re nal stroma, routine morphological examinations and counts of karyocyte were made , the expression of collagen type Ⅰand type Ⅲ were also detected by immunohist ochemical staining,then, semi-quantified by EIG image analysis system. Results A progressive fibrosis was observed in renal stroma of the mod el. 12 h after operation, counts of karyocyte increased markedly i n renal stroma in groups of treatment and pretreatment with glycyrrhizin compar ed with that in sham-operation group（P<0.05）,and decreased distinctly compared with that in saline treated group（P<0.01）, but no significant di fference was found between treatment group and pretreatment group（P>0.05） ;3 d after operation, in group treated or pretreated with glycyrrhizin. The e xpression of collagen type Ⅰ increased markedly compared with that in sham-o peration group（P<0.01） in renal stroma, but decreased notably compare d with that in saline treated group（P<0.01）,while, there was not any diffe rence between treatment group and pretreatment group（P>0.05）. The expressi on of collagen type Ⅲ in renal stroma was almost the same as that of coll agen type Ⅰ. Conclusion Glycyrrhizin has some therapeutical bu t no preventive effect to experimental obstructive nephropathy in rats.
ABSTRACT
Objective To investigate the protective role of glycyrrhizin on experimental obstructive nephropathy in rats. Methods The model of obstructive nephropathy was induced by unilateral uretera l ligation in rats and the animals were sacrificed at 12 h and 3,14,28,56 d resp ectively after operation. Specimens were taken from the cortex of kidney. In re nal stroma, routine morphological examinations and counts of karyocyte were made , the expression of collagen type Ⅰand type Ⅲ were also detected by immunohist ochemical staining,then, semi-quantified by EIG image analysis system. Results A progressive fibrosis was observed in renal stroma of the mod el. 12 h after operation, counts of karyocyte increased markedly i n renal stroma in groups of treatment and pretreatment with glycyrrhizin compar ed with that in sham-operation group（P<0.05）,and decreased distinctly compared with that in saline treated group（P<0.01）, but no significant di fference was found between treatment group and pretreatment group（P>0.05） ;3 d after operation, in group treated or pretreated with glycyrrhizin. The e xpression of collagen type Ⅰ increased markedly compared with that in sham-o peration group（P<0.01） in renal stroma, but decreased notably compare d with that in saline treated group（P<0.01）,while, there was not any diffe rence between treatment group and pretreatment group（P>0.05）. The expressi on of collagen type Ⅲ in renal stroma was almost the same as that of coll agen type Ⅰ. Conclusion Glycyrrhizin has some therapeutical bu t no preventive effect to experimental obstructive nephropathy in rats.