ABSTRACT
Objective The objective of this study were to investigate the effects of miR-219 on cell proliferation,invasion and metastasis and the correlation between PRKCI and miR-219 expression in tongue squamous cell carcinoma. Methods The lu-ciferase reporter gene assay was used to verify the predicted target gene. The expression of PRKCI in tongue squamous cell carcinoma cells overexpressed exogenous miR-219 was detected by qRT-PCR and Western blot. Finally,the reverse effects of PRKCI on the proliferation,clone formation,migration and invasion ability were examined in stable overexpressing miR-219 tongue squamous cell carcinoma(TSCC)cells by MTT assay,cell plate cloning assay,scratch assay and Transwell chamber assays. qRT-PCR assay was used to determine the expression of PRKCI gene and miR-219 in tongue squamous cell carcinoma tissues,and the relationship be-tween PRKCI and miR-219 was further analyzed. Results The bioinformatics analysis predicted that the downstream target gene of miR-219 was PRKCI. The double luciferase reporter gene assay showed that miR-219 was able to reduce the fluorescence activity of the wild type PRKCI reporter vector. In addition,qRT-PCR and Western blot also showed that miR-219 could down-regulate the expression of PRKCI in TSCC cells. MTT results showed that overexpression of PRKCI could reverse the inhibitory effect of miR-219 on the proliferation of TSCC cells,and further demonstrated that the overexpression of PRKCI could reverse the inhibitory effect of miR-219 on the proliferation, invasion and metastasis of TSCC cells by cell plate cloning, scratch and Transwell experiments. Conclusion MiR-219 plays a role in inhibiting tumor by directly targeting PRKCI and negatively regulating the expression of PRK-CI. miR-219 was negatively correlated with PRKCI expression in tongue squamous cell carcinoma.
ABSTRACT
Objective To investigate the expression of miR -219 and its role in tongue squamous cell carcinoma(TSCC).Methods The relative expression of miR -219 in the 26 samples of surgically resected paired TSCC and normal tumor -adjacent tissues was detected by qRT -PCR.The expression of miR -219 in SCC-15 and normal oral mucosa cell were also tested by qRT -PCR.The miR-219 mimic was transferred into SCC-15 cell.Cell proliferative rate and the ability of colony formation were analyzed .The abilities of migration and invasion in SCC-15 cell were evaluated by Transwell test .Results Expression of miR -219 was signifi-cantly lower in TSCC tissues(P<0.001)and SCC-15(P<0.05)in comparison with normal tumor adjacent tis-sues and normal oral mucosa cell.The abilities of proliferation(P<0.05),colony formation(P<0.05),migratio-nand invasion(P<0.05)were inhibited in miR-219-transferred SCC-15 cells.Conclusion The expression of miR-219 was downregulated in TSCC .The miR-219 could inhibit cell proliferation ,colony formation ,migra-tion and invasion in TSCC .