ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between glutathione S-transferase (GST) M1 and T1 genotypes and endometriosis risk (EM).</p><p><b>METHODS</b>Polymerase chain reaction (PCR) technique was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA isolated from the blood samples of 68 Han Chinese women with endometriosis and 28 without endometriosis.</p><p><b>RESULTS</b>The frequencies of GSTM1 and GSTT1 null genotypes in women with endometriosis were 0.721 (49/68) and 0.779 (53/68), respectively, and in women without endometriosis were 0.429 (12/28) and 0.321 (9/28), respectively. There was a significant difference with regard to the frequencies of GSTM1 and GSTT1 null genotypes between the women with and without endometriosis (P < 0.01). Furthermore, the frequencies of GSTM1 and GSTT1 null genotypes were significantly higher in the patients with stage III and IV endometriosis [0.731 (38/52) and 0.788 (41/52), respectively] than in women without endometriosis (P < 0.01), and the frequency of GSTT1 null genotype was statistically higher in patients with stage I and II endometriosis [0.75 (12/16)] than in the women without endometriosis (P < 0.01). No correlation between GSTM1 and GSTT1 null genotypes and age, induced abortion or dysmenorrhea was detected in this study (P > 0.05).</p><p><b>CONCLUSION</b>GSTM1 and GSTT1 null genotypes may be risk factors for the development of endometriosis.</p>
Subject(s)
Adult , Female , Humans , Case-Control Studies , Endometriosis , Genetics , Pathology , Genotype , Glutathione Transferase , Genetics , Risk FactorsABSTRACT
Objective To investigate the influence of genital tract cytomegalovirus and chlamydia infection on the incidence of tubal pregnancy. Methods Ninety five women with tubal pregnancy (study group) and 42 women with ovarian cysts (control group) were selected for this study. Serum cytomegalovirus (CMV) IgM was identified by enzyme link immunosorbent assay (ELISA). Cervical secretions, endometrium, and salpinx tissue were tested for CMV gH gene and chlamydia heat shock protein (HSP) gene by nest polymerase chain reaction (PCR) and PCR respectively. Results CMV IgM was positive in 14 (15%) women with tubal pregnancy, and 1 (2%) in the control group. CMV gH gene was detected in 18 women (19%) and chlamydia HSP gene in 25 (26%) of the tubal pregnancy group, and 2 (5%) and 2 (5%) in the control group respectively. There were significant differences between the study and the control group ( P
ABSTRACT
5 mm was 63 6%. There is a significant difference between in endometrial thickness with