ABSTRACT
Objective To investigate the relationship between intelligence impairment and interictal epileptiform discharges spreading in mesial temporal lobe epilepsy(mTLE)patients. Method We assessed 145 patients diagnosed as mTLE and their general materials, analyzed the relationship between intelligence impairment and interictal epileptiform discharges spreading. Results ①Patients with mTLE with longer disease course and higher frequencies of epilepsy tended to have a severe impairment in the total intelligence quotient (IQ), verbal intelligence quotient (vIQ) and performance intelligence quotient (pIQ). ② IQ of was negatively correlated with the condition that interictal epileptiform discharges spreading to the ipsilateral central and parietal region in patients with left lesion; pIQ was negatively correlated with the condition that interictal epileptiform discharges spreading to the ipsilateral frontal region, while positively correlated with the condition that interictal epileptiform discharges spreading to the ipsilateral occipital region in patients with right lesion. Conclusion ①Intelligence impairment of mTLE patients is related with courses and frequencies.②Total IQ is more severely impaired by interictal epileptiform discharges spreading to the ipsilateral central and parietal region in left mTLE patients, and the pIQ is more severely impaired by interictal epileptiform discharges spreading to the ipsilateral frontal region in right mTLE patients.
ABSTRACT
Objective To investigate the effects of different concentrations of ozone on nigra oxidative stress and apoptosis-related protein expression in rats with Parkinson's disease (PD).Methods Thirty-two adult male SD rats aged 2-3 months and weighted 180-220 g were randomly divided into 4 groups (n=8):PD group,PD+ozone 20 group (given ozone 20 mg/L),PD+ozone 50 group (given ozone 50 mg/L) and control group; rats in the former three groups were given right striatum injection of 15 μg 6-OHDA to induce PD models,and rats in the control group were given the same volume of normal saline; four weeks after PD inducement,mixed gases of 5.0 mL of oxygen and ozone (20 mg/L and 50 mg/L,1 mg/kg) were given to rats in the PD+ozone 20 group and PD+ozone 50 group,respectively,for 15 d.Then,tissues were obtained from nigra; the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),and the content of malondialdehyde (MDA) were measured by using chromometry; the protein expressions of B cell lymphoma/lewkmia-2 (Bcl-2) and Bcl-2 associated X protein (Bax) were detected by using Western blotting.Results As compared with those in the control group,the GSH-Px activity was significantly decreased and the MDA content was significantly increased in PD group,PD+ozone 20 group and PD+ozone 50 group (P＜0.05); the GSH-Px activity (6.98±0.60 vs 9.37±0.41、10.04±0.59) was significantly increased and the MDA content(2.31±0.54 vs 1.85±0.29、1.79±0.39) was significantly decreased in PD+ozone 20 group and PD+ozone 50 group as compared with those in the PD group (P＜0.05).Western blotting indicated no significant differences of protein expressions of Bax and Bcl-2 among the four groups (P＞0.05).Conclusion Ozone can improve antioxidation capability of rats with PD by increasing the activity of GSH-PX,while it does not affect the expression levels of Bax and Bcl-2.