ABSTRACT
OBJECTIVE：To optimize the formulation of Curcumin （CUR）transethosomes（CUR-TEs）. METHODS ：The contents of CUR in CUR-TEs were determined by HPLC. CUR-TEs were prepared by injection method. Using comprehensive score of encapsulation efficiency and drug loading as index ，based on signal factor test ，Box-Behnken design-response surface method was used to optimize and validate the formulation. The property of CUR-TEs prepared by the optimal formulation was investigated. RESULTS：The optimal formulation of CUR-TEs was as follows as lecithin of 4％，CUR of 0.13％，1，2-propylene glycol of 25％，tween-80 of 1％. Results of validation test of optimal formulation showed that comprehensive score of encapsulation efficiency and drug loading of CUR-TEs was 93.04±2.16，relative error of which to predicted value （91.19）was 2.03％. The encapsulation efficiency of CUR-TEs prepared by optimal formulation was （91.17±1.35）％，and its drug loading was （0.94± 0.02）％. The particle size was （190.64±15.97）nm with polydispersity index of 0.086±0.007，and Zeta potential was （－12.74± 1.60）mV. CONCLUSIONS ：The optimized formulation of CUR-TEs is stable ，feasible and repeatable ，with good stability.
ABSTRACT
An HPLC method for simultaneous determination of chlorogenic acid and mangiferin in original medicinal materials and decoction pieces of Pyrrosiae Folium was developed. The assay was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column eluted with a mobile phase consisted of acetonitrile and 0.5% phosphoric acid solution in gradient elution at a flow rate of 1.0 mL x min(-1). The column temperature was set at 25 degrees C. The detection wavelength was 320 nm. The results showed that The linear ranges of chlorogenic acid and mangiferin were 5.2-130 mg x L(-1) (r = 0.9999) and 1.2-18 microg x mL(-1) (r = 0.9999), and the average recoveries (n=6) were 97.9% (RSD 1.9%) and 99.6% (RSD 2.9%), respectively. The method was simple, reproducible and valid. It can be used for quality evaluation and control of original medicinal materials and decoction pieces of Pyrrosiae Folium.
Subject(s)
Chlorogenic Acid , Chromatography, High Pressure Liquid , Plants, Medicinal , Chemistry , Reproducibility of Results , XanthonesABSTRACT
Objective To establish a HPLC method to determine the content of acteoside in Conghuang Bushen Capsule. Methods The HPLC method was employed with a column of Hypersil ODS2(4.6 mm? 250 mm,5 ? m) and a mobile phase of acetonitrile-methanol-1 % HOAc(13 ∶ 9 ∶ 78),the detection wavelength being at 334 nm,flow rate being 1.0 mL/min and the column temperature being at 40 ℃ . Results The linear range was 0.108 5 ? g ～ 0.650 7 ? g,r=0.999; 1.The average recovery of acteoside and RSD were 98.42 % and 1.89 %,respectively. Conclusion The present method is simple,rapid and reproducible. It can be used for quality control of Conghuang Bushen Capsule.