ABSTRACT
OBJECTIVE:To establish a method for the content determination of baicalin in Yangyin mixture. METHODS:HPLC was performed on the column of SunFire C18 with mobile phase of methnol-water-phosphoric acid(43∶57∶0.2,V/V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 280 nm, column temperature was 30 ℃,and the volume injection was 10 μl. RESULTS:The linear range of baicalin was 0.055 75-1.115 μg(r=0.999 9);RSDs of precision,stability and reproducibility test were lower than 2%;recovery was 95.39%-98.44%(RSD=1.19%,n=6). CONCLUSIONS:The method is simple,rapid and accurate,and suitable for the content determination of baicalin in Yangyin mixture.
ABSTRACT
Objective: To establish the determination method of Psoralen and Isopsoralen in Gengninping Capsules.Methods: YWG-C 18 column was used and Methanol-Water (40∶60) was used as a mobile phase. The detective wavelength was 245nm.Results: This method showed a good linear relationship. The average recoveries were 99.3% for Psoralen(RSD was 1.3%, n=6) and 99.3% for Isopsoralen (RSD was 3.0%, n=6).Conclusion: This method is simple, rapid, accurate and can be used as the quantitative analysis method for this preparation.