ABSTRACT
To evaluate the plasma levels of methionine cycle intermediates: S-adenosylmethionine [SAM] and S-adenosylhomocysteine [SAH]. lipid peroxides marker: malondialdehyde [MDA]. uric acid and cellular fibronectin [cFn] in preeclamptic patients and their relations to the severity of disease. This study included 35 preeclamptic primigravid women; 19 mild and 16 severe preeclampsia- Eighteen age-matched healthy primigravidas were chosen as control group. Plasma levels of adenosylmethionine [SAM], S-adenosylhomocysteine [SAH], lipid peroxides marker: malondialdehyde [MDA], uric acid and cellular fibronectin were compared between normal pregnant, mild and severe preeclamptic pregnant women. High Performance Liquid Chromatography equipped with a reversed-phase column-C 18, and UV detector at 254 nm was used to separate SAM and SAH. Serum uric acid and MDA levels have been assayed by colorimetric methods. Serum cellular fibronectin was estimated by ELISA. Statistical analysis was performed using Mann Whitney u test and Spearman correlation analysis. Plasma levels of SAH, MDA. and cellular fibronectin were significantly increased in mild and severe preeclamptic groups compared with control group [P<0.05]. Also. these parameters were significantly higher in severe preeclamptic group than mild preecpalmatic group [P<0.05]. However, uric acid levels showed a significant increase in severe preeclamptic group compared with mild preeclamptic and control groups [P<0.05] while insignificant difference was observed between mild preeclamptic and control group [P>0.05]. SAM levels were significantly decreased only in severe preecplamsia than control [P<0.05]. Moreover. there were positive correlation between cellular fibronectin and SAH, MDA and uric acid. Disturbed methionine cycle intermediates: s-adenosylhomocysteine and s-adenosylemethionine. lipid peroxides. uric acid and fibronectin are important factors in the pathogenesis of preeclampsia and are directly related to its severity. Furthermore, cellular fibroneccin correlated positively with SAH, MDA, and uric acid suggesting [that these parameters have a primary role in endothelial dysfunction