ABSTRACT
The aim of this work was to assess the chronic effects of caffeine intramuscular administration on some metabolic functions of the liver, glycogen and protein contents of the muscle compared with the histological study of the liver of adult male white mice [Mus musculus]. After 7 days intramuscular injection of caffeine [60 mg/kg body weight] to mice [Mus musculus], it was found that glycogen content of liver and muscle was significantly decreased as compared to the control group together with significant increase in the blood and tissue glucose. The insulin level was also significantly increased. These metabolic changes were continued for 45 days and proved histochemically using Best's carmine stain. After 45 days, the hepatocytes showed marked depletion of glycogen content. The total serum and liver protein showed significant increase as compared to the control group after 7 and 45 days together with disturbed some liver function tests as serum glutamic oxaloacetic transaminase [SGOT], serum glutamic pyruvic transaminase [SGPT] and alkaline phosphatase [AP] which showed significant increase after 7 and 45 days intramuscular caffeine injection. The chronic use of caffeine in pharmaceutics should be restricted as it was proved to cause some hepatocellular and muscular metabolic disturbances