ABSTRACT
p38mitogen activated protein kinase (MAPK) inhibitors provide a novel approach for the treatment of inflammatory disorders. A series of fifteen pyrazolyl urea derivatives (-) were synthesized and evaluated for their p38MAPK inhibition and antioxidant potential. Compounds-andshowed low micromolar range potency (ICvalues ranging from 0.037 ± 1.56 to 0.069 ± 0.07 µmol/L) compared to the standard inhibitor SB 203580 (IC= 0.043 ± 3.62 µmol/L) when evaluated for p38MAPK inhibition by an immunosorbent-based assay. Antioxidant activity was measured by a 2,2'-diphenyl-1-picryl hydrazyl radical (DPPH) free radical scavenging method and one of the compounds,, showed better percentage antioxidant activity (75.06%) compared to butylated hydroxy anisole (71.53%) at 1 mmol/L concentration. Compounds-andshowed promisinganti-inflammatory activity (ranging from 62.25% to 80.93%) in comparison to diclofenac sodium (81.62%). The ulcerogenic liability and lipid peroxidation activity of these compounds were observed to be less in comparison to diclofenac sodium. These compounds also potently inhibited the lipopolysaccharide (LPS)-induced TNF-release in mice (IDof-= 19.98, 11.32 and 9.67 mg/kg, respectively). Among the screened compounds, derivativewas found to be the most potent and its binding mode within the p38MAPK is also reported.