1.
Indian J Med Microbiol
; 2015 Apr-Jun; 33(2): 290-292
Article
in English
| IMSEAR
| ID: sea-159548
ABSTRACT
Cell culture models for hepatitis B virus (HBV) remain the mainstay for screening and testing the efficacy of anti‑hepatitis B virus agents. Gradient‑based ultracentrifugation followed by Southern Blotting is used for hepatitis B virion estimation in cell culture; this method has several limitations. We report the development of an assay using a commercially available HBsAg‑ELISA plate for immunocapture followed by real‑time PCR for quantification of hepatitis B virions in cell cultures. This assay is rapid, highly sensitive (50 copies/reaction) and highly specific for virion‑associated DNA. In addition, the assay requires only 20 μL of supernatant, allowing scaling down of transfections.
2.
Article
in English
| IMSEAR
| ID: sea-159507