ABSTRACT
The components present in the essential oils extracted from Anaphalis margaritacea (L.) Benth., Salvia leucantha Cav. and Thymus linearis Benth. were identified by GC and GC/MS analysis. The main compounds present in the oils were aromadendrene (20.0%) in S. leucantha, Phellandrene (12.5%), α-thujene (12.0%) in A. margaritacea and thymol (50.0%) in T. linearis. The antibacterial activity of all the three oils against E. coli, P. aeruginosa, S. aureus and S. typhi, has been studied. In the antibacterial activity S. typhi is the most resistant bacteria to all the tested oils whereas essential oil of T. linearis showed highest antibactericidal activity against S. aureus in diffusion method.
ABSTRACT
BACKGROUND: Cancer is a multifactorial disease. Repetitive cumulative damage of cellular organelles by oxy-free radicals are few of the important causative factors. AIM: To assess the role of oxidative stress in the laryngeal cancer patients in Indian population. SETTING AND DESIGN: Case control study. MATERIALS AND METHODS: Level of malondialdehyde (MDA) as a marker of oxidative stress was examined in large cohort of control (50) and laryngeal carcinoma patients (155) from North India. Both the controls and laryngeal carcinoma patients were smokers. RESULTS: In control healthy subjects MDA levels were 0.102+/-0.07 (0.080- 0.303, 95% CI) n mol/ml, as compared to 0.329+/-0.16 (0.124-0.354, 95% CI) n mol/ml in the cases of laryngeal carcinoma patients. Three times higher serum MDA levels indicated that there was significant oxidative stress in the subjects having laryngeal carcinoma lesions. In addition patients with secondaries were having MDA levels of 0.4+/-0.02 (0.391-0.408 95% CI) n mol/ml, as compared to 0.57+/-0.03 (0.558-0.582 95% CI)n mol/ml in group of patients without secondaries. These two values were statistically significant as compared to control values (P < 0.01). CONCLUSION: These findings suggest that in case of laryngeal carcinoma patients, there is increase in the level of oxidative enzyme MDA. The oxidative stress might be due to the modulation of pro-oxidant or anti-oxidant systems in laryngeal carcinoma.