ABSTRACT
<p><b>OBJECTIVE</b>To investigate the association of MspI polymorphism of Cytochrome P4501A1 (CYP1A1) gene and smoking to the susceptibility to coronary artery disease (CAD).</p><p><b>METHODS</b>The genotypes of CYP1A1 MspI site were detected using the methods of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in 349 cases with CAD and 404 non-CAD as controls. CAD diagnosis was confirmed by coronary angiograms. Genetic risk of CYP1A1 genotypes was analyzed by smoking index (SI).</p><p><b>RESULTS</b>The frequency of the predominant homozygotes TT, heterozygotes TC and the rare homozygotes CC in CAD group were not different with that of the controls (chi(2) = 3.224, P = 0.200). But in the smokers, the frequency of CC in CAD group was higher than that of non-CAD group (P = 0.002), while its odds ratio was 3.142 [95% confidence interval (CI) 1.481 - 6.668]. The odds ratio of genotype CC and heterozygote TC was 2.215 (95% CI 1.087 - 4.510) in the low dose cigarette smoking group, and was 1.407 (95% CI 0.709 - 2.791) in the high dose cigarette smoking group.</p><p><b>CONCLUSION</b>Both MspI polymorphism of CYP1A1 gene and smoking exposure promote the development of CAD.</p>
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Coronary Artery Disease , Genetics , Cytochrome P-450 CYP1A1 , Genetics , Gene Frequency , Genetic Predisposition to Disease , Genotype , Polymorphism, Genetic , Smoking , GeneticsABSTRACT
AIM: To establish a mouse model of capsaicin-induced cough and a record device for cough. METHODS: The cough reflect in mice was induced with 0.l mmol·L-1 capsaicin by aerosol, and the number of cough was recorded by a Medlab system. RESULTS: The number of cough in mice after provoked by capsaicin aerosol was 16.8 ± 5.0 (n = 65) within 3 min. The cough reflect was inhibited by codeine pretreatment ig. ID50 values (95% confidence limits) of codeine was 9.69 (7.56 - 12.42) mg · kg-1. While pretreatment with ephedrine, a non-antitussive drug, 2.0 and 4.0 mg · kg-1 ig had no inhibitory effect. CONCLUSION: Capsaicin-induced cough model of mouse is a useful antitussive drugs screening model. The result of experiment objectively can be observed by the cough record device.