ABSTRACT
<p><b>OBJECTIVE</b>To identify the volatile components in rat urine after oral administration of "Wu-Hu-Tang" (WHT).</p><p><b>METHOD</b>GC-MS technique was applied to analyzing urine samples.</p><p><b>RESULT</b>Eighteen components were detected in the WHT-treated rat urine other than the corresponding control. Among them, 14 components were identified, and 7 were also found in the extract of WHT.</p><p><b>CONCLUSION</b>The above detected components might be derived from WHT, and some of them are effective components of WHT.</p>
Subject(s)
Animals , Male , Rats , Administration, Oral , Cyclohexenes , Drug Combinations , Drugs, Chinese Herbal , Pharmacokinetics , Ephedrine , Urine , Gas Chromatography-Mass Spectrometry , Monoterpenes , Urine , Oils, Volatile , Chemistry , Plants, Medicinal , Chemistry , Rats, WistarABSTRACT
A rapid, simple and sensitive method was establised for the evaluatiou of Chinese traditional medicine "Joufenaan" by HPLC. Sample of "Joufensan" was extracted by solvent, and the extraction analysed by HPLC using an inertsil ODS column with a solvent system, acetonitrile-water-phospboric acid-sodium dodecyl sulfate(38:62:0.1:0.5) at 40℃ and UV detector. The new method was reliable for the evaluation of ephedrine aud strychnine in "Joufensan".