ABSTRACT
We examined the effects of polyphenols on migratory activity of neutrophils into the hydrogel and their production of reactive oxygen species (ROS). Origonol and lychee polyphenol were diluted to medium in wide-range concentrations. Each solution was added on hydrogel, and the mixture of blood and luminol was layered on it in each tube. The ROS production was measured by luminol-dependent chemiluminescence, whereas the cell count in the hydrogel was quantified as migratory activity of neutrophils. The migratory activity of neutrophils was not affected, whereas ROS production of neutrophils was significantly decreased above 100 μg/ml, but increased below the concentration.<br>
ABSTRACT
<b>Objective:</b> The overproduction of reactive oxygen species leads to oxidative stress, which is related to lifestyle-related disease and cancer. Although antioxidants are considered as one of the countermeasures to oxidative stress, it is necessary to develop the assessment methodology for the antioxidant capacity which is closer to the inner body conditions. In this study, we examined antioxidant actions of plant extracts by using newly-developed neutrophil activity measurement system.<br> <b>Method:</b> Lemon verbena, Green tea, Camellia japonica and Antiallerge<sup>®</sup> were used as plant extracts, and were diluted to medium in wide-range concentrations. Each solution was added on modified Mebiol Gel<sup>®</sup> (hydrogel), and the mixture of blood and luminol were set onto the hydrogel in each tube. The amount of reactive oxygen species were measured by luminol-dependent chemiluminescence, whereas the cell count in the hydrogel was quantified as migratory activity of neutrophils.<br> <b>Result:</b> Lemon verbena and Green tea significantly inhibited reactive oxygen species in a concentration-dependent manner, whereas Camellia japonica and Antiallerge<sup>®</sup> did not. The migratory activity of neutrophils was not affected by those plant extracts. Also, damaged cells were not detected.<br> <b>Conclusion:</b> It was suggested that Lemon verbena and Green tea scavenged reactive oxygen species without causing cell death of neutrophils. This new method for measuring neutrophil activities can be applied not only for assessing the status of inflammation and oxidative stress but also as the screening system for predicting the effectiveness of antioxidant and anti-inflammatory substances.<br>
ABSTRACT
We analyzed the relation between subjective fatigue by hard exercise and stress hormone and cytokine data. The subjects are eight healthy men who had exercise experience. In this experiment, hard exercise was carried out in the first place. After two-hour rest, a performance test was carried out. The blood test was conducted 4 times (before exercise, after exercise, rest, and a performance test). Subjective fatigue was indicated using visual analogue scale (VAS). After the hard exercise and performance test, VAS increased and showed the significant difference. Lactic acid, adrenaline, noradrenaline, growth hormone, IL-6, and IL-8 increased after each exercise and showed the significant difference. Subjective fatigue and the value of lactic acid, and IL-6 showed significant positive correlations. These results suggest that lactic acid, and IL-6 may become an index of fatigue in this exertion model.<br>
ABSTRACT
The purpose of this study was to investigate moderate endurance exercise-induced changes in oxidative stress in different phases of the menstrual cycle. We used saliva samples which can be collected non-invasively. The resting oxidative stress levels determined by oxidation-reduction potential (ORP) were significantly higher in the menstrual phase than the follicular and luteal phases. The ORP significantly increased 30 minutes after exercise started and at the end of the 60 minute exercise session in the follicular phase. The menstrual cycle may affect oxidative stress levels at rest among young women, whereas moderate endurance exercise is likely to increase oxidative stress in the follicular phase.<br>