Protein chromatographic study on adult ascaris lumbricoides, ascaris vitulorum and toxocara canis

Chromatographic analysis using Sephacryl S.300 was done on protein extract of adult Ascaris lumbricoides, A. vitulorum and Toxocara canis worms. Six fractions were separated from both Ascaris species and nine from T. canis. Fractions revealed a variety of molecular weights in relation to the three species of worms that ranged from 6,000 - 70,000 daltons. Crude extract of T. canis showed cross reaction with both species of Ascaris sera using double gel diffusion, while no reaction was obtained using a partially purified antigen

Age-related rate of seropositivity of antibody to giardia lamblia in different age groups in Cairo

Two hundred and twenty normal individuals were subjected to Giardia lamblia antibody IgG analysis. All subjects were of low socioeconomic standard using Indirect Immunoflourescent Antibody Test [IFAT]. Accordirg to age the study persons were divided into 4 groups: group I with age in between 6 to 35 months, group II with age inbetween 3 years and less than 6 years, group III with age group ranged between 6 years and 16 years old and group IV with age above 16 years. The Seropositivity to Giardia infection was [37%] in all age groups, while it was 23%, 52%, 32% and 40% in each of the four age groups respectively. It is a noteworthy that infants after 12 months of age showed seropositivity to Giardia infection which meant that they caught actual infection in this early period of life while infants younger than 12 months were all seronegative to Giardia antibody. Different patterns of age associated acquisition of antibody levels were noted in the study groups. Infants and children showed a progressive acquisition of antibody titre which arrived to the highest level at the adult age group > 16 years that was significantly higher than other age groups