Response of green synthesized drug blended silver nanoparticles against periodontal disease triggering pathogenic microbiota

Objective: Evidences for microorganisms to predominate periodontal disease, alteration of human pathogenshifted microbiota by Erythrina fusca, and antimicrobial response potentiation by ecofriendly silvernanoparticles (AgNPs) intended present study to perform green synthesis of AgNPs and evaluate againstperiodontal disease triggering pathogenic microbiota.Material and Methods: Present study involved green synthesis of AgNPs using Erythrina fusca leavesaqueous extract (EFLAE), followed by optimization, characterization, stability, and evaluation of antimicrobialpotential of biosynthetic AgNPs against periodontitis causing pathogenic human microflora (Pseudomonasaeruginosa, Escherichia coli, Streptococcus pyogenes, and Staphylococcus aureus).Results: The AgNPs green synthesis success was based on brown coloration and surface plasmon resonancesignal at 433 nm. UV-Visible spectrometry driven optimization determined 5 mM AgNO3 concentration, 1:9EFLAE and AgNO3 volumetric ratio, pH 7, 60°C temperature, and 2 hours’ time as parametric requirementsfor AgNPs biosynthesis. Stability studies revealed signal appearance between 415 and 424 nm supportingAgNPs stability. Characterization studies recognized shifted and broadened Fourier transformed infrared bandsof AgNPs revealing silver capping by biochemical moieties of EFLAE; AgNPs size below 32 nm in fieldemission scanning electron microscopic micrograph; X-ray diffraction signals at 38.95, 44.97, 64.92, and78.97 representing 111, 200, 220, and 311 AgNPs silver cubic structure planes; and elemental silver 83.66%,carbon 11.87%, and oxygen 4.47% in energy-dispersive X-ray spectrum. Optimized and characterized biogenicAgNPs, when evaluated against periodontal disease-causing pathogenic microflora using well diffusion method,exhibited maximum inhibitory zone (in mm) against Bacillus cereus (13 and 18), followed by P. aeruginosa (11and 19), E. coli (10 and 18), and S. pyogenes (9 and 15) in 50 and 100 µg/ml administered dose.Conclusion: Present study concludes that biogenic AgNPs synthesized using EFLAE possess high inhibitionpotential against periodontitis triggering pathogens (E. coli, B. cereus, S. pyogenes, and P. aeruginosa) ofmicrobiota, and recommends EFLAE as potential source for AgNPs green synthesis

Antioxidative effect of Melastoma Malabathticum L Extract and Determination of its Bioactive Flavonoids from Various Location in Malaysia by RP-HPLC with Diode Array Detection.

A rapid and specific RP-HPLC method with DAD at 30C was developed and validated for the determination of quercetin (QN) and kaempferol (KMP) as marker compounds in the extract of Melastoma malabathrichum leaves. Separation was performed using Hypersil GOLD C8 column with acetonitrile - 0.1% phosphoric acid (40:60v/v) as a mobile phase with DAD detection at 370 nm. The developed method showed satisfactory reproducibility and recovery and the method was successfully applied in the determination of QN and KMP as marker compounds in MM leaves collected from different parts of Malaysia and was found to be simple, rapid and efficient. The antioxidant activity of MM of 3 different solvent extracts (methanol, ethyl acetate, chloroform) were evaluated using β-carotene bleaching method. A variation in antioxidant activities, ranging from 44.41% to 83.28% was observed and the antioxidative potency of the 3 different solvent extracts was comparable to that of butylated hydroxytoluene (BHT). The results showed that MM extracted by different solvents exhibited varying degree of antioxidant properties.