ABSTRACT
Aims: To Analysis of genetic variability in Tulsi (Ocimum sanctum) genotypes by using RAPD & SSR Markers.Place and Duration of Study: Department of Plant Biotechnology at K.K.Wagh College of Agricultural Biotechnology, Nashik.Methodology: Ocimum tenuiflorum Linn., commonly known as Tulsi, is an aromatic plant with significant traditional and medicinal value. To assess the genetic diversity and relatedness of six Tulsi genotypes (Krishna, Ram, Lavangi, Pandharpuri, Daisil, and Kapoori), molecular techniques were employed. The genotypes were collected from Nagarjuna Medicinal and Aromatic Plant Park at Dr. P.D.K.V. Akola. DNA isolation was performed using alcohol fixation without liquid nitrogen, and the genotypes were analyzed using RAPD and SSR primers for molecular characterization.Results: Genetic diversity analysis of six Tulsi genotypes (Krishna, Ram, Lavangi, Pandharpuri, Daisil, and Kapoori) was performed using RAPD and SSR markers. Five RAPD primers produced 15 bands, with 11 bands showing polymorphism (73.3%) and 4 bands showing monomorphism (26.7%). The PIC value ranged from 0.28 to 0.49 (average: 0.40). Four SSR primers generated 9 bands, with 8 bands showing polymorphism and 1 band showing monomorphism. The PIC value ranged from 0.24 to 0.57 (average: 0.39). The Jaccard coefficient revealed moderate to high similarity in RAPD (0.40 to 0.73) and SSR (0.44 to 0.88) analyses. The UPGMA dendrogram separated the genotypes into two main clusters. Cluster 1 included Krishna, Lavangi, Ram, Pandharpuri, and Daisil Tulsi, while cluster 2 consisted of Kapoori Tulsi. The SSR dendrogram also formed two clusters, with Krishna, Lavangi, Ram, Daisil, and Kapoori genotypes in cluster 1, and Pandharpuri Tulsi showing dissimilarity and forming cluster 2.
ABSTRACT
Pearl millet has a place with sort Pennisetum, and family Poaceae. Pearl millet is the most broadly developed grain crop in Asia and Africa representing close to half of the worldwide millet creation. In India, pearl millet is the fourth most broadly developed food crop after rice, wheat, and maize. We used molecular techniques to investigate the genetic diversity and relatedness of six genotypes viz:ICMV155, Dhanshakti, PC-612, Sampada, HHB-67, and HHB-197.the six genotypes was collected from National agriculture research project Aurangabad. The present study was conducted at the Department of Plant Biotechnology at K. K. Wagh College of Agricultural Biotechnology, Nashik.DNA was isolated by fixing a sample in alcohol without using liquid nitrogen; six genotypes were analyzed through SSR primers to determine the extent of molecular characterization. PCR amplification using 10 SSR primers generated a total of 111 no. of bands were scored corresponding an average of 11 bands per primer with 77 bands showing polymorphism (67%) and 34 bands showing monomorphism (30%). The PIC value ranged from 0.35 to 0.68 with an average of 0.4. Jaccard’s coefficient based on SSR analysis 0.38 to 0.90. The dendrogram wasconstructed using the UPGMA method. It has two main clusters Cluster-1 consisting of C1- HHB 67, V6-ICMV-155, C2- HHB 197 and C4- Sampada. Cluster-2 comprised C3-Dhanshakti and V5-PC 612 as an out group. C2- HHB 197 and C4- Sampada genotypes have the highest similarity coefficient 0.76. Among all the genotypes, C1- HHB 67 and V5- PC 612 was found most diverse as it separated from all other genotypes at a very low similarity coefficient of 0.6. The identified markers can prove useful for identification of diverse germplasm and future DNA fingerprinting studies.