ABSTRACT
Objective: valuating the vitamin B[1] [thiamine] on glucose homeostasis and Glycosylated hemoglobin A1 [HbA1c] in Alloxan induced diabetic rat model
Study Design: Experimental study
Place and Duration of Study: This study was conducted at the Animal House, Isra University Hyderabad from September 2014 to March 2015
Materials and Methods: 60 adult albino rats were divided into four groups; Group 1. Controls, Group 2. Rats receiving thiamine added diet, Group 3. Diabetics rats on normal diet and Group 4. Diabetic rats receiving thiamine added diet. Alloxan [120 mg/kg] was introduced intraperitoneally to induce diabetes. Vitamin B1 was given orally at 1.6 g/kg body weight for 12 weeks. Venous blood was taken from tail vein by small bore cannula at the baseline and after 12[th] week. Blood glucose and HbA1c were detected at baseline and after 12[th] week. Data was saved in proforma and analyzed on SPSS 22.0 using paired student t-test at 95% confidence interval
Results: Blood glucose and HbA1c levels were found statistically significant in groups 1 vs. 3 [p=0.0001], 1 vs. 4 [p=0.0001], 2 vs. 3 [p=0.0001], 2 vs. 4, [p=0.001] and 3 vs. 4 [p=0.024] at the end of experiment period. Significant improvement in blood glucose and HbA1c was noted in the vitamin thiamine treated rats
Conclusion: Vitamin thiamine improved the blood glucose homeostasis and reduced Glycosylated Hemoglobin A1 effectively in experimental rats. It is recommended to supplement diabetic subjects with vitamin thiamine
ABSTRACT
Objective: To study the effects of Allium sativum extract [ASE] on blood lipoproteins and blood indices in Wistar albino rat model
Study design: Experimental study
Place and Duration of Study: This study was conducted at the Animal House, Isra University Hyderabad from September 2014 to June 2015
Materials and Methods: 80 albino rats were divided into 4 groups; Group 1- Controls [Placebo 0.9% isotonic saline], Group 2- ASE 100 mg/kg, Group 3- ASE 200 mg/kg and Group 4- ASE 300 mg/kg were given orally for 30 days. Blood sample was collected by cardiac puncture. Statistical analysis was performed on SPSS 22.0 by one way ANOVA and post Hoc Duncan test at 95% confidence interval
Results: Triglycerides, total cholesterol, LDLc and HDLc showed statistically significant differences among groups [p =0.0001]. High dose fed ASE showed significant reductions in TAG, TC and LDLc and a rise in HDLc. Also the blood indices showed improvement in ASE treated rats [p=0.001]
Conclusion: The Allium sativum extract reduces triglycerides, total cholesterol and low density lipoproteins and increases high density lipoprotein [HDLc]. Blood indices were also improved in high dose rats