ABSTRACT
Objective: The objective of this study was to evaluate current susceptibility of pathogenic isolates of Staph. aureus against imipenem, methicillin and vancomycin
Methodology: Antibacterial activity was performed using disc diffusion technique
Results: The average zone values were compared by using ANOVA and result found to be significant with p-value <0.001. Highest zone values were found to be 31.36+/-5.14 for imipenem followed by methicillin 23.77 +/- 6.53. The lowest zone value were found to be for vancomycin i.e; 14.76 +/- 4.30. Imipenem was the found to be the most effective drug as 91.67% isolates were susceptible to imipenem. While high degree of resistance was observed among isolates against vancomycin [83.33%] and methicillin [70.83%]
Conclusion: Increase in resistance demands the development and rational use of new, safe and effective therapeutic agents
ABSTRACT
Ashwagandha [Withania somnifera] is rich in bioactive compounds and has so many beneficial properties
Objective: The project was designed to evaluate its current susceptibility against pathogenic organisms in comparison with antibiotics used now-a-days
Methodology: Roots of the Withania somnifera plants were collected, washed, dried, and group to powder. The extraction procedures were done using Chloroform in multiple phases. Antibacterial activity was done against pathogenic strains of E. coli and staphylococcus aureus isolated from the patient's specimen by the Kirby Bauer Technique
Results: The average zone values against E. coli isolated were found to be Withania somnifera [28.15+/- 2.39], followed by Ciprofloxacin [19.3 +/- 9.30], Cefotaxime [16.46 +/- 9.86], and Gentamicin [12.57+/- 2.19]. Withania somnifera was giving significantly higher zone than all the three drugs. Difference was significant as all the cases were with p-values <0.001. In all the cases of E.coli isolates, Withania somnifera was found to be effective and no resistance was found. Whild sensitivity of Ciprofloxacin, Cefotaxime, and Gentamicin, was 17.50%, 15%, and 2.50% respectively. The average zone values against Staphylococcus aureus isolates were found to be Imipenem [31.36 +/- 5.14], followed by Withania somnifera [29.16 +/- 2.21], Methicillin [23.77 +/- 6.53], and Vancomycin [14.76 +/- 4.30]. Withania somnifera and Imipenem were giving significantly higher zone than other two drugs with p-values <0.001. In all cases Withania somnifera was effective against Staphylococcus aureus isolates. Methicillin and Vancomycin showed no response in 58.33% and 16.67% cases respectively, showing complete resistance. Vancomycin was the most resistant and Withania somnifera was the most sensitive one
Conclusion: Results showed efficient antibacterial activity of Withania somnifera root extract against Staphylococcus aureus and Escherichia coli. Withania somnifera showed highest zone of inhibition in culture plates. Results were compared by using ANOVA and Chi-square
ABSTRACT
Objective: the objective of this study was to evaluate current susceptibility of pathogenic isolates of E coli against ciprofloxacin, cefotaxime and gentamicin
Methodology: antibacterial activity was performed using Kirby Bauer Technique
Results: the average zone values were compared by using ANOVA and result found to be significant with p-value <0.001. Highest zone values were found to be 19.31+/-9.30 for ciprofloxacin followed by cefotaxime 16.46+/-9.86. The lowest zone value was found to be for gentamicin i.e. 12.57+/-2.19. Resistance against ciprofloxacin and cefotaxime were 82.5% and 85 % respectively. While high degree of resistance was observed among isolates against gentamicin [97.5 %]
Conclusion: increasing resistance against ciprofloxacin and cefotaxime demands coordinated monitoring of activity and rational use of these antibiotics and development of new, safe and effective therapeutic agents