ABSTRACT
Objective: To develop a simple, cost-effective, and efficient medium by using sugarcane bagasse (SB) as abase material to replace the conventional Murashige and Skoog (MS) medium.Materials and Methods: Water extracts of SB along with some macronutrients and plant growth regulators weregelled with 0.7% agar-agar powder. Nodal segments of Gentiana kurroo were used as explants and inoculatedin the medium and placed in a growth chamber under standard conditions of light and temperature. Out of thetested combinations of plant growth regulators, 0.5 mg/l each of kinetin (KN) and 6-Benzylaminopurine (BAP)showed the excellent shoot multiplication and proliferation rate on the bagasse medium with the same potentialas on the MS medium with an average of 5–6 shoots/explant. In vitro rooting was obtained on half strength MSmedium supplemented with IBA (0.5 mg/l) with an average length of 7–8 cm and 20–25 roots/explant. Theplants were hardened in a mixture of clay loam and farmyard manure in 1:1(w/w) with 70%–80% survival ratewithout any phenotypic aberrations.Conclusion: The results from the present investigation indicate that SB can be used as a cost-effective substituteof MS medium for in vitro propagation of G. kurroo.
ABSTRACT
Lavandula angustifolia (Family Labiates) is a medicinal herb found in Mediterranean area. It is a well known herb in ayurvedic system of medicines and has traditionally been used to treat disorder of liver, fever and several conditions including infertility, infection and anxiety. There are few reports on tissue culture of Lavandula angustifolia that too mainly on micropropagation. Present study explored an in vitro micropropagation of Lavandula angustifolia. In vitro callus formation was established by using nodal segments on Murashique and Skoog, (1962) medium (MS) supplemented with IAA at 0.1mg/l, l BAP at 0.002mg/l and 2-4D at 0.2mg/l, significantly recorded complete callus formation after 6 weeks of incubation at 25±1ºC. The callus was allowed for organogenesis and then shoot multiplication was carried out at 4 concentrations of BAP (0.5, 1, 2 and 1mg/L) and IAA (0.5, 0.5, 0.5 and 1mg/L) on MS medium. The shoot regeneration medium for shoot multiplication and proliferation with higher number of shoots was recorded at 0.5mg/l of IAA and 2.0mg/l BAP. However, the growth was very steady and originating from the base. MS medium without any growth regulators tabulated the tallest shoot length of 35 mm, but the shoots were clustered not properly differentiated.