ABSTRACT
To investigate the effect of blood perfusion difference on oxidant status, mice were trained by a 7-week running program. Two days after the last training session, mice were exercised for 60 minutes at the same training intensity. Changes in the concentration of thiobarbituric acid reactive substance (TBARS), as an index of lipid peroxidation, in intestine, kidney and muscle, were studied in trained mice immediately (0 h), 3 h and 24 h after the running exercise and in unexercised control group. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and xanthine oxidase (XO) were determined in these tissues. Tissue SOD activities were unaffected by the exercise. Muscle GPx activity increased after exercise (0 h and 3 h group, P < 0.01) and returned to control levels at 24 h, but there was not any significant difference in intestinal and renal tissues. Renal tissue XO activity could not be determined. There was not any significant difference among groups in intestinal tissue XO activity. The activity of XO was decreased only in skeletal muscle at 0 h (P < 0.05). TBARS levels of exercised groups were higher than control in muscle (P < 0.01). Intestinal TBARS levels decreased at 0 h (P < 0.05), than reached to control level. Renal TBARS levels of 0 h and 24 h group was higher than control (P < 0.01, P < 0.01 respectively). The results show that a long distance running exercise may cause lipid peroxidation damage in skeletal muscle and kidney.
Subject(s)
Animals , Antioxidants/metabolism , Glutathione Peroxidase/metabolism , Intestines/metabolism , Kidney/metabolism , Lipid Peroxidation/physiology , Male , Mice , Muscle, Skeletal/metabolism , Physical Exertion/physiology , Superoxide Dismutase/metabolism , Thiobarbituric Acid Reactive Substances/metabolism , Xanthine Oxidase/metabolismABSTRACT
Total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) and HDL-C/TC levels are important in determining the risk of coronary heart disease. The serum lipids and lipoprotein levels of regularly training sportspersons and non-sporting controls were determined and compared with each other to investigate the effects of exercise and sex on these factors. HDL-C levels of male and female training groups were higher than those of corresponding non-sporting groups (respectively P < 0.01, P < 0.001). The sportswomen's HDL-C levels were higher (P < 0.05); and TC, TG, and LDL-C levels were lower. (P < 0.001) than those of sportsmen's levels. The non-sporting women's TC and TG levels were lower than those of non-sporting men's levels (P < 0.001). HDL-C/TC ratio of active females was higher than that of control females (P < 0.01). The corresponding difference in males was also significant. We conclude that physical activity and sex have effects on risk factors for cardiovascular disease.