Questionnaire Surveys for Evalution of Quality of Life and Problems in Patients with Orthotopic Neobladder after Cystectomy / 日本農村医学会雑誌

Questionnaire survey were conducted three times to evalute the social life and functional problems in patients with orthotopic neobladder after cystectomy. A total of 34 responses were collected froml9 patients with a mean age of 66 years at diagnosis. The patients with orthotopic neobladder were found to be troubled with prolonged urinary incontinence such as nocturnal enuresis and stress incontinence. The problems was deteriorating the quality of life of the patients.

Analysis of Two-dimensional Electrophoresis Gel Pattern of Stress Proteins induced by Moxibustion and Electroacupuncture Stimulation by Image Analyze System / 全日本鍼灸学会雑誌

Urethan-anesthetized rats were electrically stimulated for 15min by inserting acupuncture needles as electrodes into the muscle of the hips (5Hz, 3-5mA). Another rats were stimulated by moxibustion (moxa, 10mg, 10 times) for 15min. Both groups of rats were sacrificed and muscular tissues were excised 3 hours after the stimulation. The proteins extracted from the homogenized and centrifuged tissues were analyzed by two-dimensional gel electrophoreses. Stress proteins (hsp) with molecular weight of 70000 (p70), 85000 (p85) and 100000 (p100) were detected in both rats stimulated by electroacupuncture and moxibustion. Each ratio of p70, p85 and p100 to control proteins analyzed by personal image analyze system was 4.5%, 17.4% and 1.0% in electroacupuncture, 6.8%, 20.8% and 1.2% in moxibustion, respectively. The tendency of the ratios in both cases was almost similar.

Analysis of stress proteins ioduced by acupuncture stimulation / 全日本鍼灸学会雑誌

We show here that stress proteins (Mr 70K, 85K and 100K) were induced by both electroacupuncture and moxibustion.<br>Rats (Wister strain, male, 250g) were cauterized with moxa on the hip, and the subcutaneous and intramuscular temperatures of the hip were kept at 45°C and at 39-40°C for 15 minutes. While, another group of rats were electrically stimulated for 15min inserting acupuncture needles as electrodes into the muscle of the hips in the depth of 1cm and using current pulse acupuncture system (5Hz, 3-5mA). Both groups of rats were sacrificed under deep anesthesia and the muscular tissues were excised 3 hours after the stimulations. The proteins extracted from the homogenized and centrifuged tissues in the two groups of rats and control rats were analyzed by two-dimensional gel electrophoreses. The heat shock proteins with molecular weight of 70000 (Mr 70K, hsp 70), 85000 (Mr 85K, hsp 85) and 100000 (Mr 100K, hsp 100) were detected in the rats stimulated by moxibustion. The stress proteins of molecular mass 70000, 85000 and 100000 similar to hsps induced by moxibustion were found in the rats stimulated by electroacupuncture.

Relationship between acupuncture and stress proteins / 全日本鍼灸学会雑誌

Rats were cauterized with moxa on the hips, and the subcutaneous and intramuscular tempetratures were kept at 45°C and at 40°C for 15min, respectively. Rats were killed by perfusion of sucrose solution under deep anesthesia and the muscle tissues of the hips were collected from the bodies at 0h, 3h and 24h after the moxibustion. The proteins were extracted from the homogenized and centrifuged tissues. Two dimensional gel electrophoreses of the proteins were carried out. Heat-shock proteins of molecular mass 70, 000 (hsp 70) and 71, 000 (hsp 71) were detected for the rat killed 3h after the moxibustion. However, no hsp 70 but hsp 71 was detected in the rats killed 0h and 24h after the moxibustion. Hsp 71 was detected in the control rat muscle as well.