ABSTRACT
Objective:To analyze the diversity of Bacteroides in simple obesity children aged 7-14 and the difference from that of normal individuals.And to explore the influence of children's simple obesity on intestinal advantage bacterium of the genus.Methods:We have collected fecal samples of 30 cases with simple obesity aged 7 to 14 years old and 30 healthy children in the Central Hospital of Shan County.All bacterial DNAs were extracted from the feces, and then Bacteroides 16s DNA specific primers was used in PCR amplification, separated by DGGE, the intestinal Bacteroides molecular fingerprints of obese children and healthy children were obtained, and the diversity and similarity were analyzed.At the same time, the weight, height, BMI, blood pressure, blood lipid, leptin, plasma adiponectin in the obesity and normal children were measured, the relationship between the obese children and the advantage of intestinal bacteria genera and its clinical significance were investigated.Results:The BMI[(25.71±3.28)kg/m 2], SBP[(121±12.4)mmHg], TG[(1.15±0.40)mmol/L], TC[(4.20±0.73)mmol/L], LEP(18.94±1.68) in the obesity group were higher than those in the normal group [BMI(17.38±3.54)kg/m 2, SBP(104±7.3)mmHg, TG(0.87±0.31)mmol/L, TC(3.37±0.86)mmol/L, LEP(11.29±2.41)](all P<0.05). The levels of HDL[(1.01±0.28)mmol/L] and ADP(4.10±2.32) in the obesity group were lower than those in the normal group [HDL(1.40±0.26)mmol/L, ADP(5.84±3.42)]( P<0.05). There were no statistically significant differences in age, DBP and LDL levels between the two groups (all P>0.05). The stripe number of Bacteroides DGGE profiles (9.33±1.37) and H index(2.07±0.16) in the obesity group were lower than those in the normal group [(17.33±2.80), (2.67±0.20)]. Cluster analysis results showed that the similarities of obese individuals in the group and compared with normal group were reduced.The S and H index of DGGE profiles in Bacteroides were negatively correlated with BMI and LEP ( P<0.01), and positively correlated with ADP( P<0.05). S, H index of the normal group were negatively correlated with LEP ( P<0.05), and had no correlation with BMI, ADP( P>0.05). S and H index of the obesity group were negatively correlated with BMI and LEP, and had no correlation with ADP ( P>0.05). Conclusion:Human intestinal flora of Bacteroides changes is related to childhood obesity, the diversity and similarity of obese children's intestinal Bacteroides species are decreased.The diversity of obesity children's Bacteroides is associated with BMI, ADP and LEP.
ABSTRACT
Objective To research the effect of neoadjuvant chemotherapy with anthracycline and taxane in early or locally advanced breast cancer and its effects on ER, PR and Her-2.Methods 120 cases of early or locally advanced breast cancer patients were selected as the research objects, according to the order of admission, the patients were divided into the observation group and the control group.The control group were given conventional chemotherapy with EC regimen (epirubicin +cyclophosphamide), while the observation group were treated with anthracyclines and taxanes.The clinical efficacy and the expression of ER, PR and Her-2 receptor in the two groups after treatment were compared.Results The total effective rate of the observation group was 73.33%, which was higher than that of the control group (53.33%) (P<0.05).After treatment, the positive expression level (0~+++) of ER receptor in the observation group were 20.00%, 15.00%, 35.00%, 30.00%, respectively, the positive expression level(0~+++) of PR receptor were 26.67%, 20.00%, 23.33% and 30.00%, respectively, were significantly better than those of the control group ( ER:31.67%, 21.67%, 28.33%, 18.33%, PR:40.00%, 25.00%, 20.00%, 15.00%) (P<0.05).But there was no significant difference between the two groups in the expression of Her-2 receptor (25.00%, 11.67%, 30.00%, 33.33% and 31.67%, 21.67%, 16.67%, 30.00%, respectively).The incidence of adverse reactions in the observation group and the control group were 6.67% and 21.67%, respectively, and there was significant difference between the two groups ( P<0.05 ) .Conclusion In the treatment of early or late stage breast cancer , anthracycline combined with taxane neoadjuvant chemotherapy has a significant effect, which can effectively improve the expression of ER and PR receptors.In addition to improve the effect of clinical treatment, and reduce the incidence of adverse reactions in a certain extent,so it can be used as a new adjuvant chemotherapy in the clinical application of the best option.