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【Objective】 To evaluate the viability of nucleic acid test(NAT) of human parvovirus B19 (HPV B19), hepatitis A virus (HAV) and hepatitis E virus (HEV) along with routine NAT for voluntary blood donors, and to analyze HPV B19, HAV and HEV prevalence in Nantong, so as to provide reference for rational blood screening programs. 【Methods】 HPV B19 DNA, HAV RNA and HEV RNA of blood donors in Nantong from November 2021 to May 2022 were detected using NAT, and serological antibody testing was performed on NAT reactive samples. 【Results】 Three HPV B19 DNA was yielded out of 3 440 blood donors, with a positive rate of 0.09%, among which 2 were negative for HPV B19-IgM and 1 was undetermined due to insufficient sample size. HAV RNA and HEV RNA were not detected in 3 440 blood donors. HPV B19, HAV, and HEV NAT were conducted simultaneously with routine HBV, HCV and HIV screening, prolonging the test reports by 20 minutes. 【Conclusion】 Although the HPV B19 DNA, HAV RNA and HEV RNA prevalence among voluntary blood donors in Nantong is low, the risk of transfusion transmitted infection still exsits and can be reduced by NAT.
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【Objective】 To evaluate the necessity and rationality of setting 0.7 CO of the gray area of HBV surface antigen reagent (ELISA) in our laboratory, and to provide the basis for the grey area setting. 【Methods】 A total of 60 samples of serum plate were repeatability tested by two kinds of ELISA HBsAg reagents. Based on the test results, C50, C5, C95 concentrations and corresponding S / CO values were calculated, and whether C5 and C95 were within C50±20% was verified. At the same time, the true positive rate and the confirmed positive rate of gray area samples revealed by two reagents were calculated. 【Results】 The confirmation results of serum plate were as follows: The C50, C5 and C95 concentrations of reagent A were 0.090 IU/mL, 0.075 IU/mL, 0.105 IU/mL. The true positive rate was 99.1%(436/440), the confirmed positive rate of grey area was37.8%(136/360). The C50, C5 and C95 concentrations of reagent B were 0.112 IU/mL, 0.091 IU/mL, and 0.133 IU/mL; the true positive detection rate was 97.1%(233/240); the confirmed positive rate of grey area was 35.8%(129/360). It was verified that C5 and C95 of reagent A and reagent B were within their respective C50±20%, and the gray areas of the HBsAg ELISA were verified to be effective. 【Conclusion】 It is necessary to set the gray area for these two HBsAg ELISA reagents in our laboratory, but the gray area value setting to 0.7 CO is unreasonable. The best gray area value was 0.75 CO in reagent A and 0.63 CO in reagent B.
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Objective:To investigate the influencing factors for postoperative deep vein thrombosis in elderly patients with inguinal hernia.Methods:The retrospective case-control study was conducted. The clinical data of 352 elderly patients with inguinal hernia who were admitted to the Affiliated Hospital of Jining Medical College from March 2018 to March 2021 were collected. There were 325 males and 27 females, aged (72±3)years. Observation indicators: (1) treatment and follow-up; (2) analysis of influencing factors for postoperative deep vein thrombosis. Follow-up was conducted using outpatient examination and telephone interview to detect occurrence of deep vein thrombosis in patients up to September 2021. Measurement data with normal distribution were represented as Mean± SD, and comparison between groups was conducted using the t test. Count data were described as absolute numbers, and comparison between groups was conducted using the chi-square test. The univariate analysis was conducted using the corresponding statistical methods based on data type. The Logistic regression model was used for multivariate analysis. Results:(1) Treatment and follow-up. Of the 352 inguinal hernia patients, there were 18 cases with irreducible hernia and 334 cases with reducible hernia. All the 352 patients underwent laparoscopic hernio-rrhaphy successfully, including 95 cases with totally extraperitoneal prosthetic, 257 cases with transabdominal preperitoneal prosthesis. The operation time and volume of intraoperative blood loss of the 352 patients were (70±7)minutes and (8.0±1.5)mL, respectively. There were 39 cases with intraoperative blood transfusion and 313 cases without intraoperative blood transfusion, 167 cases with common patch, 185 cases with light patch. The compression time of operative area, time to first out-of-bed activities, duration of postoperative hospital stay of the 352 patients were (19.7±2.9)hours, (5.6±1.8)hours, (3.0±1.9)days, respectively. All the 352 patients were followed up for 6 months after operation. During the follow-up, there were 7 patients with deep vein thrombosis. (2) Analysis of influencing factors for postoperative deep vein thrombosis. Results of univariate analysis showed that age, body mass index, smoking history, alcoholism history, hypertension, chronic obstructive pulmonary disease, coronary heart disease, cerebrovascular disease, varicose veins, intraoperative blood transfusion, time to postoperative first out-of-bed activities, duration of postoperative hospital stay were related factors for postoperative deep vein thrombosis in elderly patients with inguinal hernia ( t=2.19, 2.06, χ2=9.86, 9.02, 7.90, 14.36, 17.12, 36.25, 28.27, 7.32, t=3.30, 3.04, P<0.05). Results of multivariate analysis showed that age, hypertension, chronic obstruc-tive pulmonary disease, coronary heart disease, cerebrovascular disease, varicose veins, intraopera-tive blood transfusion, time to postoperative first out-of-bed activities, duration of postoperative hospital stay were independent influencing factors for postoperative deep vein thrombosis in elderly patients with inguinal hernia ( odds ratio=4.32, 5.95, 6.44, 15.85, 9.63, 7.61, 9.88, 7.27, 7.96, 95% confidence intervals as 0.92?8.72, 4.45?12.74, 1.74?19.46, 3.97?36.84, 4.95?16.26, 2.65?18.03, 3.50?21.47, 1.48?16.37, 2.08?14.73, P<0.05). Conclusion:Age, hypertension, chronic obstructive pulmonary disease, coronary heart disease, cerebrovascular disease, varicose veins, intraoperative blood transfusion, time to postoperative first out-of-bed activities, duration of postoperative hospital stay are independent influencing factors for postoperative deep vein thrombosis in elderly patients with inguinal hernia.
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【Objective】 To analyze and evaluate the application of intelligent voice call technology (IVCT) in the recall of lost blood donors, so as to provide references for the intelligent development of blood donor recruitment. 【Methods】 SMS, human telephone and IVCT were used to recall the lost blood donors from 2016 to 2018, and the recall results were statistically analyzed. 【Results】 20 406 blood donors were recalled from August 20 to December 30, 2020 through 1 029 human telephones, 7 571 automated calls, and 11 806 SMSs, and 4.81%(32/665), 3.44%(51/1 484) and 1.17%(138/11 806) of them responded. There was no significant difference in the recall effect between human telephones and automated calls. The one-year-lapsed blood donors responded about 3 times of those lapsed for 2~4 years (5.97/2.02). 【Conclusion】 The application of IVCT can make up for the shortages of high demand, much interference and long time consuming of human telephones, reduce the labor intensity, improve the efficiency of blood donor recruitment, and promote the recall of lapsed blood donors.
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Objective To investigate the efficacy of laparoscopic percutaneous common bile duct exploration (LPCBDE) with internal draining tube placement for the treatment of cholelithiasis. Methods The clinical data of 962 patients with choledocholithiasis who were admitted to the No. 451 Hospital of PLA were retrospectively analyzed. A self-made internal draining tube was placed in the common bile duct and duodenum to drain bile internally. The correct position of the internal draining tube was comfirmed by injecting water into and draining water from duodenum. The internal draining tube was pulled out with the help of duodenoscope at 30 days after the operation. Results LPCBDE with internal draining tube placement was successfully performed on 864 patients. Forty-two patients were transferred to open surgery, and 56 patients were transferred to receive LPCBDE with T-tube drainage. The mean operation time was (36 ± 18) minutes (range, 20-72 minutes), and the length of postoperative hospital stay was (6.6 ±2.1)days. Two patients were complicated with retroperitoneal abscess and they were cured by puncture and drainage, 32 patients were complicated with bile leakage and they were cured by conservative treatment. A total of 862 patients were followed up by B ultrasound at 30 days after the operation. The internal draining tube which was confirmed in the common bile duct was extracted with duodenoscope in 603 patients; the internal draining tube which was drawn back in 1 patient was removed with endoscopic sphincterotomy ( EST); the internal draining tube was removed naturally in 258 patients. The follow-up period ranged from 1 to 3 years, 26 patients had recurrent cholelithiasis and they were treated by EST. Conclusions LPCBDE with internal draining tube placement is a safe and minimally invasive method for the treatment of cholelithiasis.
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Extra-luminal flow hollow fiber membrane oxygenator (ELFHFMO) has been widely applied in cardiopulmonary bypass (CPB) and extracorporeal membrane oxygenation (ECMO) or extracorporeal life support (ECLS) because of its advantages over other types of MO. But its low hemocompatibility and durability are the major problems in clinical application that even have limited its wide application. In this paper, we reviewed the recent researches on how to improve the hemocompatibility and durability of ELFHFMO.
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Humans , Coated Materials, Biocompatible , Extracorporeal Membrane Oxygenation , Heparin , Oxygenators, MembraneABSTRACT
Objective To probe into the variation of CT values of surface enhanced layers of nasopharynx wall,intensified regularity and choosing the best scanning time.Methods 138 patients received conventional plus enhanced CT scan and 58 cases of them received delayed and repeated scans.Results 83% of the cases were correspondent to the surface of nasopharynx and had manifest linear enhancement.After giving contrast injection,it reached 97% at the 74 second.All CT values of the cases were enhanced in the phase of 92～344 second.Conclusion The ratio of linear enhancement is manifestly high,which is very helpful to the CT diangosis of NPC restricted to or under the mucosa.The best acquisition time should be just before or after the 74 second,correspondent to the surface of nasopharynx.The enhancement lasted long.
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Objective To observe the effect of laparoscopic surgery for patients with acute gallstone pancreatitis(AGP). Methods The clinical data of 134 patients suffered from AGP treated with laparoscopy surgery since 2000 in our hospital were analyzed retrospectively.Results All the patients were treated with laparoscopic surgery successfully. Among them, 21 cases were treated with laparoscopic cholecystectomy (LC);113 cases with LC and exploration of common bile duct,induding 75 cases received opening the pancreatic capsule and placement of irregation tubes for postoperative washing the abdominal cavity during the same operation. One hundred and tweent-six cases(94.0%) cured, 6 cases(4.5%) died, 2 cases( 1.5%) discharged themself. Conclusions Laparoscopic surgery in the treatment of early stage of AGP can get good results and improve the prognosis remarkably.It is worth to be used widely.
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Objective To study the optimal laparoscopic procedure and its indication for remove of common bile duct stone. Methods Analysis was made on the clmical data of 124 cases of laparoscopic choledocholithotomy and T tube drainage in our center.Results 82 patients underwent the improved laparoscopic procedure, alternation to open operation in 4 cases (4.9%),and the mean operating time was (80?30) min. While 42 patients were operated with traditional laparoscopic method,changing to open operation in 6 cases (14.3%),and the mean operating time was (170?40) min . The improved method could shorten the operation time and reduce the open operation rate significantly than traditional method did (P
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Objective To explore the value of application of choledochofiberscopy in the diagnosis and treatment of the extrahepatic bile duct disease, and the effect on reducing the incidence of the postoperative residual stone in biliary ducts. Methods According to the case history and ultrasonography,if the common bile duct(CBD) diseases suspected,the CBD was explored by intraoperative choledochofiberscope(IOCF). During the procedure,a biliary passage mirror inducer apparatus and biliary tract probe which were manufactured by ourselves were used. Results During LC,IOCF was performed on 385 cases of the 10 396 LC cases,and possitive findings were dicovered in 102 cases(26.49%). Among those positive patients, 67 cases belonged to stricture of the lower biliary tract; 5 cases were Mirizzi syndrome; 2 cases were carcinoma of the periampulla; 1 case was primarily carcinoma of the bile duct; 1case was ascarisis of the biliary system. Conclusions IOCF is a good inspect technique with high success rate and clear image of bile duct, it can discover the common duct diseases which are difficult to be diagnosed through the routine examination.At the same time, it can provide the locative and qualitive diagnosis, determine reasonable methods of operation,and effectively provent postoperative complications.