Enhanced porcine interferon-alpha production by Pichia pastoris by methanol/sorbitol co-feeding and energy metabolism shift / 生物工程学报

Porcine interferon-alpha (pIFN-alpha) fermentative production by recombinant Pichia pastoris was carried out in a 10-L bioreactor to study its metabolism changes and effects on fermentation under different inducing strategies, by analyzing the change patterns of the corresponding metabolism and energy regeneration. The results show that the specific activities of alcohol oxidase (AOX), formaldehyde dehydrogenase (FLD) and formate dehydrogenase (FDH) largely increased when reducing temperature from 30 degrees C to 20 degrees C under pure methanol induction, leading significant enhancements in methanol metabolism, formaldehyde dissimilatory energy metabolism and pIFN-alpha antiviral activity. The highest pIFN-alpha antiviral activity reached 1.4 x 10(6) IU/mL, which was about 10-folds of that obtained under 30 degrees C induction. Using methanol/sorbitol co-feeding strategy at 30 degrees C, the major energy metabolism energizing pIFN-alpha synthesis shifted from formaldehyde dissimilatory energy metabolism pathway to TCA cycle, formaldehyde dissimilatory pathway was weakened and accumulation of toxic intermediate metabolite-formaldehyde was relieved, and methanol flux distribution towards to pIFN-alpha synthesis was enhanced. Under this condition, the highest pIFN-alpha antiviral activity reached 1.8 x 10(7) IU/mL which was about 100-folds of that obtained under pure methanol induction at 30 degrees C. More important, enhanced pIFN-alpha production with methanol/sorbitol co-feeding strategy could be implemented under mild conditions, which greatly reduced the fermentation costs and improved the entire fermentation performance.

Clinical value of contrast-enhanced TVS in the assessment of invasion depth of endometrial cancer / 中国基层医药

Objective To discuss the diagnostic value of contrast-enhanced transvaginal sonography TVS in the diagnosis of myometrial invasion grade of endometrial cancer. Methods Total twenty-eight cases were studied by contrast-enhanced TVS,which were proved pathologically endometrial cancer in our hospital. The diagnosis of myometrial invasion grade of endometrial cancer by contrast-enhanced TVS were compared with pathologic results based on FIGO. Results The total coincidence of contrast-enhanced transvaginal sonography in detecting the depth of myometrial invasion was 78.6%. In the evaluation of no myometrial invasion,it showed the sensitivity of 71.4%,specificity of 85.7% and coincidence of 62.5%. In evaluation of superficial myometrial invasion,the sensitivity,specificity and coincidence was 73.7% ,77. 8% ,87. 5% ,respectively. The sensitivity in detecting deep invasion was 100. 0% ,the specificity was 96.0% ,while coincidence was 75.0%.There was no significant difference among the coincidence of various depth of myometrial invasion by contrast-enhanced TVS. Conclusion Contrast-enhanced TVS was valuable in the diagnosis of myometrial invasion grade of endometrial cancer.

Testicular microlithiasis and its relation to varicocele on ultrasound findings of symptomatic men / 中国医师进修杂志

Objective To study testicular microlithiasis(TM)on scrotal ultrasound findings in re-lation to the incidence of varicocele in males with scrotal symptoms.Methods Ultrasound finding of 8351 cases aged 40 days to 88 years with scrotal symptoms were reviewed.Results TM was found in 170 pa-tients(2.04%)and varicocele Was found in 1449 patients(17.35%)of symptomatic individuals.Varicocele Was found in TM and non-TM were 15.88%(27/170)and 17.38%(1422/8181)respectively,statistical anal-ysis revealed that there was no close relationship between TM and varicocele(x2=0.26,P>0.05)in symp-tomatic population. Conclusion TM is a comnlofl disease and has no direct correlation with varicocele.