ABSTRACT
Objective Serum pharmacochemistry was performed to screen the bioactive constituents of Resina Draconis.Methods Based on HPLC fingerprints of Resina Draconis,the migrating constituents absorbed into blood were determined by comparing the HPLC fingerprints of extraction of Resina Draconis(ERB),herb serum sample,and control serum sample,and with the help of LC-MS/MS.Results Six compounds absorbed into blood were detected,five of them were original in form which were 3,4′-dihydroxy-5-methoxystilbene,cochinchinenin B,4′-hydroxy-4,2′-dimethoxy-dihydrochalcone(a new compound),cochinchinenin A,and loureirin B,respectively.The other might be the original constituent or the metabolite.Conclusion The six constituents absorbed into blood are possible bioactive components of Resina Draconis in vivo.Further research will help clarify the bioactive constituents and mechanisms of Resina Draconis.
ABSTRACT
Object To study the technology of supercritical CO_2 extraction of effective components from Dragon's Blood which has the inhibiting function on ?-glucosidase. Methods The influence of the extraction pressure, temperature, time and fluid velocity on surplus extraction rate and the improvement of inhibition was mainly discussed. Results The optimal experimental parameters were 15 MPa extraction pressure, 45 ℃ extraction temperature, 30 kg/h fluid velocity and 2 h extraction time. Conclusion The results show that SFE-CO_2 can enormously increase the surplus extraction rate and the inhibitory activities on ?-glucosidase.