Combined analysis of transcriptomics and metabolomics revealed complex metabolic genes for diterpenoids biosynthesis in different organs of Anoectochilus roxburghii / 中草药·英文版

OBJECTIVE@#Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii, a precious medicinal plant, are important active components. However, due to the lack of genetic information on the metabolic process of diterpenoids in A. roxburghii, the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear. This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of A. roxburghii by combining analysis of transcriptomics and metabolomics.@*METHODS@#The differences in diterpenoid accumulation in roots, stems and leaves of A. roxburghii were analyzed by metabonomic analysis, and its metabolic gene information was obtained by transcriptome sequencing. Then, the molecular mechanism of differential diterpenoid accumulation in different organs of A. roxburghii was analyzed from the perspective of gene expression patterns.@*RESULTS@#A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in A. roxburghii. There were 38, 34, and 18 diterpenoids with different contents between roots and leaves, between leaves and stems, and between roots and stems, respectively. Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified, and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA, GA20ox, GA3ox, GA2ox, and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids. In addition, 14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis. The expression of genes such as GA2ox, MAS, CPA, GA20ox and GA3ox might be activated by some of the 14 transcription factors. The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors.@*CONCLUSION@#This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in A. roxburghii, which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of A. roxburghii.

Sonographic assessment for breast augmentation by repeated injection of low volume of autologous fat / 中华医学美学美容杂志

Objective To assess the absorbance rate of the fat after the operation of breast augmentation of the repeated injection of low volume, using ultrasonic imaging method. Methods Thirty three patients were injected with low volume of autologous fat (50-60 ml per time) to bilateral breasts for 1-5 times and breast sonographic examination was performed to evaluate the grafted fat tissues. The thickness of the retromammary fat layer before and after each injection was measured to calculate the absorbance index. Results The 264 points of breast were measured in this study. The fat was distributed in the retro-mammary fat layers at 224 points of the breast and in the pectoralis major muscle layer at 32 points of the breast, and the others distributed in the mammary gland layer.The average thickness of the retromammary fat layer increased gradually from 0.2 cm before the first operation to 1. 0 cm after the fifth operation. The average absorbance index one month after each operation was 34 %-66 %. Conclusion The present study demonstrates that breast augmentation by repeated autologous fat graft with low volume injection at each time is applicable and satisfactory and that breast ultrasound is an accurate and simple method to e-valuate the absorbance index.

Ultrasonographic features in complications of cosmetic augmentation with autologous fat obtained by liposuction / 中华超声影像学杂志

Objective To evaluate the ultrasonographic (US) features and evolution of breast fat necrosis after cosmetic augmentation with autologous fat obtained by liposuction, to help distinguish fat necrosis from more ominous breast masses. Methods Breast sonography was performed on 38 patients underwent bilateral breast augmentation by autologous fat injection to evaluate the grafted fat tissues in interval of 3-6 month after the operation. Observations in follow up sonography included the sizes,positions, shape,echogenicity,margin features, calcifications and evolutions of the suspicious nodules in the breasts. Results Seventy-six nodules occurred in 25 of the 38 patients were detected after the fat graft. Among the 76 nodules,52 were cystic(68. 4%) ,8 were complex(10. 5%) and 16 were solid(21.1%). The analysis of the predominant features of the nodules sonographic appearances were as follows: all the nodules had no flow signal, 66 (86. 8%) had clear margins,54(71. 1%) had regular shapes,52 were cystic(68. 4%) ,63(82. 9% ) had no calcifications, 10(13. 2%) had egg-like calcifications,74(97. 4% ) had no halo,and the positions of the solid components in 8 complex nodules move following the change of the detected body position. There were 7 nodules with fat necrosis removed surgically and confirmed by pathology. Conclusions Breast ultrasound is an accurate and simple method to follow up the temporal changes of the fat nodules after autologous fat injection. It may help to avoid unnecessary biopsies.

A study of the pour blood and the oxygen metabolic in tissue for extra-artery fascia flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>The aim of the work was to study the change for blood pour into the artery fascial flap and oxygen metabolic in tissue that knew the process of the flap.</p><p><b>METHODS</b>We do the artery fascial flap in the rabbit, then measured the skin capillary blood flow and the content of the MDA.</p><p><b>RESULTS</b>The content of the MDA rise when the blood flow descends in the flap, but it lives well.</p><p><b>CONCLUSIONS</b>That the content of the MDA rise as time the blood flow descend in the flap is a gradual process of the whole flap from tip to end, the flap will not appear necrosis until the MDA reach a rather degree.</p>

Experimental study on the toxic effects of hydrophilic polyacrylamide gel / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the safety of Hydrophilic polyacrylamide gel (HPAG) through an animal experiment.</p><p><b>METHODS</b>After HPAG was injected underneath the skin of SD rats, tissue specimens were taken for general and histological examinations. The cytotoxicity was evaluated by agar coverage and MTT method.</p><p><b>RESULTS</b>It was determined that the cytotoxicity was over level-two. The toxicity to kidney was obvious. The local histological reaction was slight and a thin fibrous membrane was formed around HPAG, which became stiff gradually. The shape and location of the injected HPAG was not stable. The HPAG could not be drawn out completely.</p><p><b>CONCLUSION</b>HPAG has obvious cytotoxicity and is not a suitable material as soft tissue implant for the bad shape and texture.</p>

Physical and psychological changes in patients with traumatic deformities during the treatment of tissue expansion / 中华整形外科杂志

<p><b>OBJECTIVE</b>This study was to investigate the somatic symptoms and sychological specialties in trauma-deformed patients during tissue expansion.</p><p><b>METHODS</b>Using our own questionnaires, fifty-seven patients were solicited to answer the questionnaires in three different treatment periods.</p><p><b>RESULTS</b>It was found that local distension, pain and tickle were the common symptoms during expansion, while distension was most specific (P < 0.01). Pain and tickle also happened before implanting and after taking out of the expander. The patients often had high expectation toward tissue expansion. They felt nervous and anxious (P < 0.01) during expansion. Their emotion and healthy self-evaluation changed obviously in different treatment periods (P < 0.01).</p><p><b>CONCLUSION</b>Local distension is the most specific symptom during tissue expansion. Nervousness and anxiety are common in patients at that time. Their emotion change from depression to happy at first, and then from happy to slight depression.</p>

Fat particle injection auto-transplantation a 10-year review / 中华整形外科杂志

<p><b>OBJECTIVE</b>To summarize clinical experiences in fat particle injection auto-transplantation during the past ten years.</p><p><b>METHODS</b>Retrospective analysis of 334 cases of fat particle injection auto-transplantation was done, and we suggested the correct method of liposuction and fat injection.</p><p><b>RESULTS</b>In this series, one patient (0.29%) had a complication, thirty one patients (9.38%) had lower survival of autogenous fat-transplantation.</p><p><b>CONCLUSION</b>In order to improve the results of fat-grafting, we must adopt the correct method of liposuction and follow the right rules.</p>

Leptin as an angiogenic factor in grainy fat transplantation / 中华医学美学美容杂志

Objective To investigate the angiogenic effect of leptin on grainy fat transplantation. Methods Grainy fat was obtained from the Wistar rat subcutaneous tissue in the abdomen. It was then mixed with 0.9 % NS and 20 ng/ml leptin and transplanted in the head subcutaneously. The transplanted tissue was taken out after 10, 20 and 40 days and was stained with AKP histochemistry and actin immunohistochemistry. The microvessel density was measured by medical imagine analysis system. The data were analyzed with SPSS software. Results The values of microvessel density in leptin groups were 0.12 ?0.04?m 2/?m 2 after 10 days and (6.83?1.86)?10 -2 ?m 2/?m 2 after 20 days. This was obviously high as compared with (1.49?0.88)?10 -2 ?m 2/?m 2 and (4.32?2.11)?10 -2 ?m 2/?m 2 in saline groups in the same observing periods ( P

Use of polypropylene mesh for abdominal defect after TRAM flap transfer / 中华医学美学美容杂志

Objective To investigate the ideal method of repair ing the abdominal defect after the TRAM flap transfer. Methods 24 cases of the abdominal defect after TRAM flap transfer were repaired by a polypropypene mesh. The anterior rectus sheaths on the pedicle were directel y sutured. Results Using of polypropylene mesh not only r epaired the abdominal defect but also achieved a superior abdominal aesthetic re sult. No ″abdominal compartment syndrome″ and infection happened d uring the ho spitalization. Mean patient follow-up was 1 year. No abdominal hernia, bulge an d laxity were encountered. The result of the abdominoplasty was continuous and t he functions of the abdomen were good. Conclusions It is an ideal method that using of polypropylene meth repairs the defect of abdomen . It keeps the integrity and the functions of the abdomen, decreases the complica tions, and at the same time gets the abdominoplasty effect.

Multi-factors analyses affecting cosmetic results after breast conservation therapy / 中华医学美学美容杂志

Objective To investigate the factors affecting the cosmetic results in the breast cancer treated with breast conservation to get the better cosmetic results.Methods Treated breasts were scored by the plastic surgeon and the patient independently.Mann-Whitney U test was used in univariate analyses whereas logistic regression was used in multivariate analyses.Results In the surgeon's evaluation, the satisfactory rate was 65.7 %.In univariate analyses,age(P=0.003),body mass index(BMI)(P=0.002),menopausal status(P=0.002),the radiation dose(P=0.022),the number of the incisions as the tumour was located in the outer-upper quadrant(P=0.036),the resection times(P=0.036),fibrosis(P=0.002),the type of surgery(P=0.002) were the significant factors affecting cosmetic results.In multivariate analyses,fibrosis,the type of surgery and the radiation dose(P=0.009,0.001,0.019) were the significant factors affecting cosmetic results.In the patient's evaluation,the satisfactory rate was 76.1%.In univariate analyses,the type of surgery (P=0.001) and fibrosis(P=0.045) were found to be significant factors. In multivariate analyses using logistic regression,the type of surgery(P=0.005) were the most significant factors.Conclusions The satisfactory rate has positive correlation between the surgeon and the patient.In the twenty-seven possible factors affecting the cosmetic results,age,body mass index,menopausal status,radiation dose,the number of the incisions as the tumour located in the outer-upper quadrant,the resection times,fibrosis and the type of surgery are the main factors affecting the cosmetic results significantly.