Bioactive compounds of thai red hot chili dip (nam prik ta dang) and effects on antioxidant activities and lipid peroxidation in rats (in vitro and in vivo models).

This study aimed to study the efficacy of Thai Red hot chili dip (Nam Prik Ta Dang) on antioxidant activity and lipid peroxidation inhibitor in vitro and in vivo models. Healthy recipe of Nam Prik Ta Dang (NPTD) was developed by Institute of Nutrition, Mahidol University used in this study. Fresh NPTD was determined for some bioactive compounds, and the antioxidant activities were evaluated by two different methods as oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP). The result showed that NPTD contained total polyphenol and also carotenoids such as beta-carotene and lutein. In vitro antioxidant activity values of NPTD was 204.3 ± 8.5 and 17.2 ± 2.7 micromole Trolox equivalent/gram for ORAC and FRAP, respectively. Freeze-dried NPTD was used for evaluating antioxidant activity and antilipid peroxidation in healthy rats. Daily administration of normal diet mixed NPTD for 2 months, serum ORAC significantly increased to compare with control group (p < 0.01). Lipid peroxidation marker as serum Thiobarbituric acid reactive substances (TBARS) significantly decreased to compare with control group (p < 0.05). In conclusion, healthy recipes of Thai red hot chili dip or Nam Prik TA Dang obtained various bioactive compounds which exhibited significantly antioxidant activities in vitro and in vivo. Moreover, daily consumption of NPTD showed the efficacy in inhibiting lipid peroxidation. Further study, it could be studied in oxidative stress condition and human clinical trials.

Antioxidant and antiplasmodial activities of curcuma longa and aegle marmelos on malaria infeced mice (in vitro and in vivo).

The purpose of this study is to evaluate the antioxidant activity of Thai Curcuma longa and Aegle marmelos, and their parasite suppressive effects on malaria infection in mouse model. The polyphenol content and antioxidant activities (Oxygen radical absorbance capacity (ORAC) and Ferric Reducing Antioxidant Power (FRAP)) were measured in crude extracts of Curcuma longa and Aegle marmelos. Seven-week old female ICR mice were divided into 5 groups randomly. First two groups served as control and placebo, the other 3 served as experimental groups with various concentrations; 20, 40 and 60 mg/kg, respectively. On day 0, 106 Plasmodium yoelii 17X (lethal) strain were inoculated to all mice. At day 1, placebo mice were given 30% ethanol. The latter 3 groups were treated with each concentration of each herb. Parasitemia was checked daily by tail snip bleed staining with Giemsa staining. Suppressive effects on day 4 were calculated. Student t-test was performed to display the difference among groups. Significantly different was justified at p <0.05. The antioxidant activities (both ORAC and FRAP) and polyphenol content of A. marmelos were higher than those of C. longa significantly. The suppressive effect on parasite in infected mice, there showed no effect of C. longa treatment, oppositely, at the doses of 20 and 40 mg/ kg body weigh of A. marmelos showed suppressive effect on infection by the parasite. In conclusion, a Thai traditional fruit, A. marmelos exhibited high antioxidant and antiplasmodial activities, this might be one of the candidates of the traditional plants for curing malaria.