ABSTRACT
Objective To explore the significance of serum carcinoembryonic antigen (CEA),carbohydrate antigen (CA) 199,CA242,and CA724 combined detection in the diagnosis of colorectal cancer.Methods The levels of CA724,CA242,CA199,and CEA in 112 cases of colorectal cancers,74 cases of colorectal benign lesion group,and 128 cases of healthy physical examination group were detected by chemiluminescence method.Results The levels of CEA,CA199,CA242,and CA724 in sera were significantly higher than those in colorectal cancer group (P ＜ 0.05),and the difference between two groups was not significant (P ＞ 0.05).In the clinical diagnosis of colon and rectal cancers,the sensitivity,specificity,and detection rate of CEA were 52.3％,93.5％,and 76.8％;CA199 was 46.9％,91.9％,and 73.6％;CA242 was 56.3％,94.6％,and 79.0％;and CA724 was 49.2％,93.0％,and 75.2％.The combined detection of CEA,CA199,CA242,and CA724 were 76.6％,96.2％,and 87.6％.Conclusions In the diagnosis of colorectal cancer,combined detection of serum CEA,CA199,CA242,and CA724 has more clinical value.than single detection,and can improve the diagnostic sensitivity and detection rate.
ABSTRACT
Objective The expression of TGF-β1 and IL-10 in the grafts of inbred mice with rejection of heart transplantation were studied and the interaction of them in the rejection of heart transplantation in inbred mice investigated.Methods Allografts were divided into 2 groups:control group (n =70),cyclosporin A-treated group (CsA group,n =70).Hearts from inbred BABL/c mice were transplanted into a cervical location in the other recipients and the survival time of the allografts was observed.The local expression of TGF-β1 and IL-10 was detected at day 1,3,7,11,14,21 by reverse transcription polymerase chain reaction(RT-PCT) respectively.Results The survival time of the allografts was (20.3 ± 1.7) days in control group,and(32.2 t 3.4) days in CsA group (P ＜ 0.01).The levels of the two cytokines expression were up-regulated in CsA group.The up-regulation of TGF-β1 was closely correlated with the survival of the grafts.Conclusion The expression and production of the two cytokines is up-regulated probably cause of administ ration of cyclosporin A,and favorite to the heart graft survival,and action of these two cytokines are probably interrelated.
ABSTRACT
In order to better match the teaching hours of clinical anesthesiology with the teaching content of modem anesthesia, the teachers must take full advantage of various supplementary teaching methods in the limited teaching hours to help enhance the students' enthusiasm in active learning, help the students concentrate on the crucial points and apply their knowledge and ultimately improve the teaching quality.