ABSTRACT
Objective:To investigate the serum levels of soluble Fas (sFas) and soluble FasL (sFasL) in elderly patients with skin cancer and its clinical significance.Methods:Eighty-two elderly skin cancer patients who underwent surgical treatment in Zhuji People′s Hospital and Zhejiang Provincial People′s Hospital from January 2014 to December 2017 were selected as the skin cancer group, and 82 healthy elderly patients were selected as the control group. The serum sFas and sFasL levels were determined by double anti sandwich enzyme linked immunosorbent assay (ELISA).Results:The serum levels of sFas and sFasL in the skin cancer group were higher than those in the control group [(21.06 ± 3.23) μg/L vs. (5.64 ± 1.18) μg/L, (14.52 ± 3.42) μg/L vs.(3.27 ± 0.96) μg/L], there were significant differences ( t=40.606, 28.679, P<0.05). The serum sFas and sFasL levels were closely related to pathological grade and lymph node metastasis. The serum levels of sFas and sFasL in patients with pathological grade Ⅲ-Ⅳ were higher than those at grade Ⅰ-Ⅱ [(23.57 ± 3.16) μg/L vs. (16.35 ± 3.62) μg/L, (17.16 ± 3.29) μg/L vs. (12.15 ± 3.58) μg/L], there were significant differences ( t=8.931, 6.173, P<0.05). The serum levels of sFas and sFasL in patients with lymph node metastasis were higher than those without lymph node metastasis [(24.09 ± 3.46) μg/L vs. (14.37 ± 3.19) μg/L, (18.17 ± 3.64) μg/L vs. (11.02 ± 3.27) μg/L], there were significant differences ( t=13.124, 9.306, P<0.05). The serum sFas and sFasL levels were not associated with age, gender, location and type ( P>0.05). The serum sFas and sFasL levels after treatment were lower in patients with skin cancer than before treatment [(7.94 ± 1.21) μg/L vs. (21.06 ± 3.23) μg/L, (5.38 ± 1.02) μg/L vs. (14.52 ± 3.42) μg/L], there were significant differences ( t=34.445, 23.191, P<0.05). Conclusions:Serum sFas and sFasL levels are elevated in elderly skin cancer patients. The serum sFas and sFasL levels are closely related to pathological grade and lymph node metastasis in elderly skin cancer patients, and the clinical efficacy can be evaluated.
ABSTRACT
Objective To determine the protein expression level of p-STAT1 and STAT1 in thyroid cancer and its correlation with clinical pathological parameters,especially with lymph node metastasis and relapse-free survival.Methods p-STAT1 and STAT1 protein were measured by immunohistochemical staining in 80 cases of primary thyroid carcinoma and matched normal controls.All samples were selected from the pathological storage in the Jiangdong 22nd Zone,of Zhuji People's Hospital.The correlation between p-STAT1,STAT1 and patients clinical pathological parameters was analyzed.74 out of 80 patients were followed up over 3 years to analyze the potential relationship between expression of p-STAT1 and STAT1 and relapse-free survival.Results The positive expression of p-STAT1 and STAT1 was 28.8％ and 90.0％ in thyroid cancer tissue versus 85.0％ and 50.0％ in normal thyroid tissue.The difference was statistically significant(P ＜ 0.05).The positve expression of p-STAT1 in stage Ⅲ and Ⅳ thyroid cancers was significantly lower than in stage Ⅰ and stage Ⅱ thyroid cancer(P ＜ 0.05).There was negative correlation between the expression of p-STAT1 and tumor stage and lymph node metastasis,while the expression of STAT1 was irrelevant.Furthermore,the relapse-free survival was 100％ in p-STAT1 positive patients,and 92.65％ in STAT1 positive group.Conclusion p-STAT1 protein level,negatively correlating with tumor stage and lymph node metastasis,down regulates in primary thyroid cancers,and may be a novel biomarker for thyroid cancer metatstasis.
ABSTRACT
Objective To develop a new type of oxygen mask, which has adjustable vent and can be removable. Methods An adjustable and removable vent below the mask was added to the conventional mask, so the problem of taking off the mask for interrupted oxygen inhalation was solved. The change of the passage of the oxygen import and export could add comfort, keep away from the operation area of the neck,chest area, etc. The new mask had a filmy hole in each side which was for gastrointestinal drainage tubes,which increased tighmess. Results The new mask improved the effect of oxygen inhalation, added comfort to patients and was convenient for medical operation. Conclusions The mask is innovative and can be widely used in clinic.