Seroprevalence of human toxocariasis [visceral larva migrans]

A total of 455 patients who fulfilled the inclusion criteria were included in the study. The enrolled patients were subjected to a questionnaire [including sociodemographic and other risk factors] and thorough clinical examination was done for the patients. Sera were collected from patients and tested for anti-Toxocara IgG antibodies using ELISA. The overall and- Toxocara sero-positive was [7.7%]. It was significantly higher than among the randomly selected 30 healthy controls. There were no significant differences between the seropositive and seronegative patients regarding age, sex, educational level and monthly family income of the patient. However, rural residence, poor house, pet's ownership and frequent contact with soil were found to be significant. Patients who had confirmed bronchial asthma were more than 2 times at higher risk of developing toxocariasis [OR, 2.33; 95% CL 1.09-4.98] than those with other clinical diagnosis [PUO, hepato-megaly or heptosplenomegaly, lympha-denopathy, neurological disorders, gastrointestinal troubles and dermatitis]. Patients with eosinophilia were at 149 times greater risk of being Toxocara seropositive compared to those without eosinophilia [OR, 148.7; 95% CI: 53.5-413.3]. Multivariate regression analysis showed eosinophilia and contact with soil were the most important predictors of toxocariasis. OD of anti-Toxocara antibodies [ELISA] was significantly positive with eosinophilia level

Effect of insect growth regulators on protein pattern of dermatophagoides farinae and its validity on inducing human alleregens

Two insect growth regulators, Methorprene and Hydroprene were evaluated as growth hormones one the common house dust mite; Dermatophagoides farinae. The adults and immature stages were treated with 4 concentrations of each regulator as contact and as diet. The data were recorded 30 and 90 days post- exposure. Both compounds significantly suppressed population growth, as compared to >/= 5% acetone controls. Methoprene was more effective than Hydroprene when given in diet by corporation bioassays but, slightly more effective for the 1st 30 days of the contact assays. A second set of contact and diet -incorporation assays was done using a 75% of Methoprene and Hydroprene to determine efficacy on D. farinae population dynamics, at weekly intervals for 13 weeks. The average number of the mites in the untreated control population was increased by more than 10 times than the original ones. One the other hand, the number of treated mites was not significantly increased. This was similar for both the contact and the diet-incorporation bioassays, although the average number of mites/container was significantly higher when the treatment was incorporated in the house dust mite diet than when applied directly as contact. Consequently, the insect growth regulators minimized the allergic efficacy of D. farinae experimental exposed mice

Zoonotic cryptosporidiosis in man and animal in farms, giza governorate, Egypt

To demonstrated the zoonotic cryptosporidiosis. A total of 189 farmers and their farm animals [200= 71 buffaloes, 59 cows, 43 sheep and 27 goats were examined. Stool samples Each stool sample was subjected to the direct wet smear method and Sheather's sugar flotation and acid-ether sedimentation techniques and stained with four stains [Modified Ziehl Neelsen, Safranin, methylene blue. Aniline carbol methyl violet and Giemsa]. A positive statistically significant correlation was observed between farmers and their farm animals infected with cryptosporidium parvum proving a zoonotic potential for infection [r = 0.819 P <0.01 for cryptosporidiosis. For detection of Cryptosporidium, 100% sensitivity was obtained when acid ether concentration technique was used and modified Ziehl-Neelsen stain revealed highest sensitivity of 92.1%