ABSTRACT
This work studied the role of parasitic infection among 85 chronic diarrheic patients in Delta region and cross-matched 20 normal controls. They were subjected to thorough history taking and clinical examination and stool examination by direct smear, formol-ether concentration, simple sedimentation, simple floatation and Kato-katz thick smear. Questionnaire sheet was obtained for each case included personal history, complaint, present as well as past history and family history. The results showed that 67.1% of patients suffered from parasites versus 20% in controls. They included giardiasis mixed with hymenolepiasis nana, ameobiasis; ascariaisis, S.mansoni, heterophyiasis, B. hominis, Taenia spp and enterobiasis respectively. Single infection represented 54.2 %, while mixed ones were 12.9% of total chronic diarrhea cases and non-parasitic causes were responsible for 32.9%. Mixed infection was common in A. lumbricoides with E.histolytica [18.18%] and H. nana with G. lambia [27.28%]. The diarrhea duration was longer in mixed infections [3 months], E.histolytica [2 months] and H.nana [1.5 months]. Commonest symptom other than diarrhea was abdominal pain mainly in mixed parasitosis. Parasitic diarrhea was more common in males than females [1.28: 1]. Chronic parasitic diarrhea was most prevalent among low social class [49 or 57.6%] followed by very low social class [20 or 23.5%], middle social class [10 or 11.7%] and finally high social class [6 or 7.1%] with significant increase in low social class as compared to high one, and most prevalent among positive cases in rural area than in urban area
Subject(s)
Humans , Male , Female , Chronic Disease , Feces/parasitology , Surveys and Questionnaires , Giardiasis , Amebiasis , Ascariasis , Social Class , Rural Population , IncidenceABSTRACT
Hepatitis C virus [HCV] is a major public health problem worldwide, which causes high rate of chronic liver disease such as liver cirrhosis and hepatocellular carcinoma. Plasma transforming growth factor Beta-1 [TGFB-1] is a member of large family of peptides, which has a major regulatory role in hepatic fibrosis and cirrhosis. The study evaluated the role of transforming growth factor Beta-1 [TGFB-1] in induction of fibrosis in liver parasites-free HCV patients with related steatohepatitis. Thirty HCV patients who were clinically and serologically positive were selected. They were diagnosed as fatty liver by abdominal ultrasonography; steatohepatitis and confirmed by histopathological biopsies examination. ELISA evaluated plasma transforming growth factor Beta-1 [TGFB-1] level. Also, 12 cross-matched subjects clinically, parasitologically and serologically free were used as a controls. The level of plasma transforming growth factor Beta-1 [TGFB-1] was highly elevated in the patients versus controls with mean +/- SD 18739.86 +/- 18539.46 and 6465 +/- 1142 respectively [P < 0.001]. The TGFB-1 level in HCV related steato-hepatitis was elevated in all grades in contrast to controls [P < 0.05], without relation between the TGFB-1 levels and steatohepatitis severity. The TGFB-1 level showed high significant difference in all stages of fibrosis in patients in contrast to controls and the TGFB-1 level was very high when fibrosis started in stage I [P < 0.01] and tended to decrease in fibrosis of stage 2 and 3 [P < 0.05]. There was highly significant positive correlation between TGFB-1 and body mass index [BMI] r = 0.774
Subject(s)
Humans , Male , Female , Fatty Liver , Liver Cirrhosis , Transforming Growth Factor beta/blood , Liver Function Tests , Body Mass IndexABSTRACT
Clean laboratory bred albino mice were used. Three mice of these were used for testing the infectivity of eggs. Twenty four mice were sacrificed at 3 weeks and 3 months, 12 each time. Immunohistochemical study which is a combination of immunological and pathological changes was performed to determine the differential population of inflammatory cells in the reaction produced to T. canis infection in early and late stages