ABSTRACT
This study was designed to evaluate the possible role of Nigella sativa L. [NS] oil, as immuno-stimulatory agent against DMBA-induced hamster buccal pouch [HBP] carcinogenesis. This study was carried-out on eighty-five male golden Syrian hamsters divided into nine main groups. In animals of DMBA-treated groups, the left buccal pouches were painted with 0.5% DMBA, 3 times/week. Animals of NS-treated groups were given 5 mgs /day of NS oil orally. Other groups were given both NS and DMBA at the same time, 3 times/week. Before animals sacrificing, blood samples were withdrawn from the orbital sinus. Both buccal pouches were surgically excised, fixed in 10% neutral buffered formaline, and processed for H and E stain. Topical application of DMBA in HBP induced immunosuppression through reduction of lymphocytes production and produced different grades of epithelial dysplasia. Administration of NS oil, significantly enhanced the immune system through increased lymphocytes production, and inhibited development of advanced dysplastic changes. These findings suggest that NS oil is a potential retarding agent of DMBA-induced HBP carcinogenesis possibly through enhancing the cell-mediated immune system
Subject(s)
Male , Animals, Laboratory , Anthracenes/adverse effects , Carcinogens , Leukocyte Count , Lymphocytes , Protective Agents , Treatment Outcome , Follow-Up Studies , Cricetinae , Models, Animal , Adjuvants, Immunologic , 9,10-Dimethyl-1,2-benzanthracene/toxicity , Plant OilsABSTRACT
This study was designed to evaluate the possible role of Nigella sativa L. [NS] oil, as chemopreventive agent against DMBA- induced hamster buccal pouch [HBP] carcinogenesis. This study was carried-out on eighty-five male golden Syrian hamsters divided into nine main groups. In animals of DMBA-treated groups the left buccal pouches were painted with 0.5% DMBA, 3 times/week. Animals of NS-treated groups, were given 5 mgs/day of NS oil orally. Other groups were given both NS and DMBA at the same time, 3 times/week for 6 weeks. The animals were sacrificed by inhalation of high dose of ether. Both buccal pouches were surgically excised fixed in 10% neutral buffered formaline, and processed for H and E and p53 immunohistochemical stains. Topical application of DMBA in HBP, produced different grades of epithelial dysplasia, and over-expression of mutant p53. Administration of NS oil, inhibited the development of advanced dysplastic changes, and decreased expression of mutant p53. These findings suggest that NS oil is a potential retarding agent of DMBA-induced HBP carcinogenesis through down-regulating mutant p53 expression