Coagulopathy: influence of Schistosoma mansoni and Hbs Ag altered equilibria in post-partum preeclamptic states

The anticoagulants, protein C [PC], protein S [PS] and thrombin-antithrombin complex [TAT] were assessed in four selected groups involving non-pregnant cases [Group I[A]IV[A]] [G I[A]; were considered as controls, G II[A]: with active schistosomiasis, G III[A]: with hepatitis B surface antigen [Hbs Ag] carrier inactive state and G IV[A]: with S. mansoni and Hbs Ag carrier inactive state], and allied cases immediately after delivery [Group I[B]-IV[B]]. A similar category of selected groups were assessed for those suffering from preeclampsia [PE] prior to delivery [Group I[C]-IV[C]]. The groups involved normal control group [G I], a group with schistosoma mansoni [G II], a group that were inactive Hbs Ag carrier [G III] and a group with schistosoma and inactive Hbs Ag carrier state [G IV]. Reduced PC, PS vs elevated TAT [G IV[A] > G II[A] > G III[A] > G I[A]] were presented in non-pregnant states. Assessed values immediately after delivery showed greater-magnitude of change in PE than those with normotensive pregnancies with significantly higher TAT. A relative decrease of PC and PS levels [G Iv[C] > G lic > G III[C] > G I[C]] compared to [G IV[B] > G II[B] > G III[B] > G I[B]] was apparent. Significant decrease of PC and PS were evident only in G Iv[C]. In conclusion, preeclamptic schistosomal cases with inactive Hbs Ag carrier state presented greater risk of thrombotic complications owing to inadequate complementary compensatory mechanism between PC and antithrombin III [AT III]

Utilization of date-seed oil in production of tetracycline and chlortetracycline by streptomyces aureofaciens ATCC-10762

In a study of date-seed oil, 7% yield of total lipid was extractable from the seed of date palm [Phoenix dactylifera]. Thin-layer chromatography revealed that phospholipids were absent in Barni date-seed lipid, but mono-, di-, triacylglycerols, cholesterol esters and fatty acids were present. Gas chromatographic analysis was carried out on the fatty acids present in the date-seed lipid. The results revealed that lauric acid [130.61 mug/ml] was the major saturated fatty acid, while oleic acid [209.41 mug/ml] was the main unsaturated fatty acid. Myristic acid [64.7 mug/ml], palmitic acid [71.8 mug/ml], stearic acid [11.9 mug/ml] and linoleic acid [59.5 g/ml] were also present. Different date-seed lipid concentrations were used as carbon source in the fermentation medium for bioformation of tetracycline [TC] and chlortetracycline [CTC]. The high titers of TC and CTC were obtained when date-seed lipid was 30 mg/ml. Date-seed lipid was more efficient than dextrin when used as a carbon source. On utilizing date-seed lipid in the fermentation process, the residual fatty acids present in the fermented medium were determined. It was found that unsaturated fatty acids were consumed by the organism at greater percentages [49.97% oleic and 49.47% linoleic acid] than the saturated fatty acids [40.74% stearic, 39.17% myristic, 38.66% palmitic and 30.02% lauric acid]. Therefore, date-seed lipid could be used efficiently as carbon source in the formation of TC and CTC fermentation media