Immune response to brucella: transfer factor from guinea pigs

Br. melitensis was isolated from human patients suffering from brucellosis. The micro-organisms were serologically identified using some serological tests, like ELISA and fluorescent tests. Using guinea pigs immunized with brucella mciro-organisms, we found that a low molecular weight substance derived from spleen cells was able to transfer sensitivity to brucella antigen, to non-immunized animals. Material obtained from spleens of sline treated guinea pigs did not transfer sensitivity to brucella antigen