ABSTRACT
Objective: To assess the effects of Manuka honey, Ocimum sanctum, Curcuma longa, and 0.2% chlorhexidine mouthwash on Streptococcus mutans and Lactobacillus acidophilus levels. Material and Methods: A randomized controlled trial will be conducted on dental students of Teerthanker Mahaveer Dental College and Research Centre, Moradabad. The study participants will be divided into four groups. Each group will have a total of 20 individuals. By using a lottery system, Group A (Manuka honey mouthrinse), Group B (Ocimum sanctum mouthrinse), Group C (Curcuma longa mouthrinse), and Group D (0.2% chlorhexidine mouthrinse) will be chosen. To match the circadian cycle, saliva will be collected at baseline and again after 2 weeks between 10 and 10.30 a.m. The sterile container will subsequently be delivered to the microbiological laboratory and processed as soon as possible to measure Streptococcus mutans and Lactobacillus acidophilus count. For 2 weeks, participants were told to use 10 mL of mouthrinse twice daily. Results: The mean oral hygiene index?simplified (OHI?S) score of all the four groups showed reduction in their scores from baseline to after the study period. For both S. mutans and L. acidophilus, there was a substantial Percentage Reduction (PR) between the prerinse and postrinse samples in all four groups. Discussion: Because quantitative actions play a crucial part in the caries disease process, the changes in microbial activity before and after administration of experimental mouthwashes were examined. Conclusion: Essential oil aqueous extracts were as efficient antibacterial mouthwashes as chlorhexidine and iodine mouthwashes
ABSTRACT
Background: Rheumatoid arthritis is a heterogenous autoimmune disorder of unknown cause with variable clinical expression. Genetic factors play an important role and likely account for about 60% of disease susceptibility and expression. The aim of this study to find out the association of CRP haplotypes in rheumatoid arthritis and their correlation with severity of the disease. Material and Methods: This was case control study where in all available patients and volunteers (only for blood samples) were recruited. Peripheral blood samples of patients were collected at Rheumatology Clinic and Medicine Department of S.P. Medical College, Bikaner in collaboration with Department of Biological Sciences, BITS, Pilani-Hyderabad during July 2009 to January 2012. 100 control subjects with no known history of disease and 135 cases were recruited as per pre-decided inclusion and exclusion criteria. A tag SNP approach captured common variation at the CRP locus and the relationship between genotype and serum CRP was explored by linear modelling. Results: Cases comprised of 98 females (Mean age 43.01+13.23 yrs) and 37 (mean age 47.4+14.9 years) males. The Control group comprised of 100 unrelated healthy controls. The cases and controls did not differ significantly for any of the clinical parameters, except for serum CRP levels. The allele distribution of rs1205 polymorphism among the studied cases and controls, which was statistical non-significant. The rs3093066 polymorphism located at the 3` position of the gene in the UTR at position number 157949723. The rs3116640 polymorphism located at 157948938 position on chromosome1 and the allele distribution of rs3116637 polymorphism among cases and controls which was also found to be monomorphic respectively. Conclusion: Extending the studies to a larger cohort will also allow genetic analyses of clinically defined endophenotypes observed in the patients of this chronic metabolic disease with attributes of autoimmune disorder and multiple symptoms in patients. Genetic studies can also impact strategies adopted for effective personalized treatment for this progressively debilitating disease.
ABSTRACT
Objective: The aim of this study was to assess the understanding and perception of Coronavirus Disease 2019 (COVID-19) among the undergraduate preclinical and clinical dental students within Pakistan. Material and Methods: An online survey was developed by the researchers and conducted amongst the undergraduate students at various dental colleges of Pakistan. A pretested questionnaire was used and distributed using social media platforms. The questionnaire consisted of demographics, health status, perception related to general hygiene, understanding and learning attitudes of dental students. Knowledge scores and the attitude responses were compared with the demographics using independent t-test, one-way ANOVA and chi-square, as appropriate. Results: A total of 800 undergraduate dental students responded to the survey, out of which 304(38%) were males and 496(62%) were female students. Students in pre-clinical years were 600(75%), while 200(25%) students were in the clinical years. The knowledge of clinical students regarding COVID-19 was statistically higher (4.71/6), compared to the students in pre-clinical years (4.49/6) (p=0.004). Both groups showed adequate knowledge regarding the route of transmission, symptoms and origin of COVID-19, and COVID-19 being a threat to life. Clinical students did not want to re-use the face mask (75%), compared to the preclinical students (68%) (p-0.048). Students in clinical years reported more awareness regarding the recommended hand washing technique during COVID-19 (p-<0.0 01). Conclusion: It can be concluded that dental students in clinical years have adequate knowledge regarding the COVID-19 as compared to the preclinical students. The study identifies all areas where preclinical and clinical students lack basic knowledge. This should be compensated by arranging different webinars and online courses to increase the understanding of both clinical and preclinical students during the pandemic.
Objetivo: El objetivo de este estudio fue evaluar la comprensión y la percepción de la enfermedad por coronavirus 2019 (COVID-19) entre los estudiantes de pregrado de odontología clínica y preclínica en Pakistán. Material y Métodos: Los investigadores desarrollaron una encuesta en línea que se llevó a cabo entre los estudiantes de pregrado de varias facultades de odontología de Pakistán. Se utilizó y distribuyó un cuestionario, previamente probado, a través de plataformas de redes sociales. El cuestionario comprendía datos demográficos, estado de salud, percepción relacionada con la higiene general, comprensión y actitudes de aprendizaje de los estudiantes de odontología. Las puntuaciones de conocimiento y las respuestas de actitud se compararon con los datos demográficos mediante la prueba t independiente, ANOVA de una vía y chi-cuadrado, según corresponda. Resultados: Un total de 800 estudiantes de odontología de pregrado respondieron a la encuesta, de los cuales 304 (38%) eran hombres y 496 (62%) eran mujeres. Los estudiantes en los años preclínicos eran 600 (75%), mientras que 200 (25%) estaban cursando años clínicos. El conocimiento de los estudiantes de clínica sobre COVID-19 fue estadísticamente mayor (4.71/6), en comparación con los estudiantes en años preclínicos (4.49/6) (p=0.004). Ambos grupos mostraron un conocimiento adecuado sobre la vía de transmisión, síntomas y origen de COVID-19, y que COVID-19 es una amenaza para la vida. Los estudiantes en fase clínica no querían reutilizar la mascarilla (75%), en comparación con los estudiantes preclínicos (68%) (p-0.048). Los estudiantes en años clínicos informaron más conciencia sobre la técnica de lavado de manos recomendada durante COVID-19 (p<0,001). Conclusión: Se puede concluir que los estudiantes de odontología en años clínicos tienen un conocimiento adecuado sobre el COVID-19 en comparación con los estudiantes en años preclínicos. El estudio identifica todas las áreas donde los estudiantes clínicos y no clínicos carecen de conocimientos básicos. Esto debe compensarse organizando diferentes seminarios web y cursos en línea para aumentar la comprensión de los estudiantes clínicos y no clínicos durante la pandemia.