ABSTRACT
In the present study three bacterial isolates namely MJS1101, MJS1102 and MJS1103 were isolated and purified from oil contaminated soil sample. Oil degradation potential of all the three isolates was evaluated by studying their growth and protein profile while they were inoculated in Minimal salt media supplemented with 5% used engine oil for a period of 10 days. The isolate MJS1102 showed maximum growth (Quantified by OD at 600nm and enumeration on Nutrient agar plates) throughout the incubation period and also concentration of protein in the flask containing MJS1102 was found to be maximum throughout the incubation period thereby by giving an indication that the isolate was able to utilize the hydrocarbons present in used engine oil as a source of carbon and energy. Percentage oil degradation by the isolate was also calculated and it was found to be 84.41%.
ABSTRACT
A highly alkaline amylase producing Bacillus megaterium was isolated from enriched soil under lab conditions. In order to enrich the soil for alkaline amylase producing Bacteria, soil was supplied with raw potatoes (substrate) and water at pH 10. Soil was left for 1month. After serial dilution five different colonies were obtained and were screened for amylase production. The culture MJS1105 showing maximum hydrolysis zone was subjected to solid state fermentation using wheat bran as substrate. The enzyme was purified using ammonium sulphate precipitation followed by dialysis. Total activity of 7.056 U was obtained at 37ºC after 96 hours of incubation and was found to have a molecular weight of 63KDa (approx.) by SDS – PAGE. The enzyme was relatively stable between pH 5 – pH 13 and at temperature ranging from 32ºC to 50ºC. Moreover, activity was enhanced by using metal ions such as Ca2+, Mg2+ and Na2+. The decline in activity was observed by adding SDS and EDTA.
ABSTRACT
The main aim of this study is that due to increasing concerns about the development of antimicrobial resistance among pathogenic bacteria, so alternative strategies are sought that do not use antibiotics to reduce pathogenic bacteria from foods and patients. Plants have been in use for thousands of years to conserve food and treat health diseases. The pericarp (peels) of Punica granatum has been commonly employed as a crude drug in Indian traditional medicine for treatment of diarrhea as well as for use as an antihelminthic, diuretic, stomachic, cardiotonic. Antibacterial properties of Punica granatum pericarp (peels) extracts (hot aqueous, methanolic and ethanolic) were evaluated against E.coli, P.aeruginosa and S.aureus using agar well diffusion method. Hot aqueous, methanolic and ethanolic extracts of Punica granatum pericap show an average inhibitory zone diameter of 23.3, 22.3 and 24.5mm respectively which indicates that ethanolic extract shows best result having ZOI greater than that of the standard antibiotic Tetracycline (20.1mm). Ehanolic extract of Punica Granatum has lowest MIC of 1.45 μg/ml showing that it is most effective as compared to MICs of other extracts.