ABSTRACT
Aims: The study was conducted to assess the synergistic poly-herbal formulation for diabetic patients to determine its three characteristics reducing excessive sugar level to normal, immune-potentiating and antioxidant. Study Design: Subjects were divided into four groups, Group I(NH), were normal healthy subjects, Group II(DI), were diabetics, group III (TTD) were tolbutamide treated diabetic patients, group IV(HFTD), were diabetic patients receiving combination herbal formulation in the, dosage of 5 g. /day for 4 weeks. Place of Study: Diabetic patients were contacted and convinced from two Government Hospitals-Hamidia Hospital, Bhopal and K. N. Katju hospital, Bhopal, (M.P.), India. Methodology: homogenous mixtures were obtained and encapsulated 500mg/per capsule. PMNL were isolated from blood and glucose level tests performed by autoanalyzer. Immune- potentiating activity was evaluated by different following methods like a ATPase sensitivity tests, cellular water content, cell, plasma membrane calcium content, Camp activity, Phaspholipase-C activity, contact angle measurement, NBT assay. Antioxidant activity evaluates by SOD and glutathione peroxidase methods. Results: Encouraging results prompt that, herbal formulation, which could be proved on excellent sugar level regulator. For efficient phagocytosis by PMNL such as membrane potential, cellular water content, calcium homeostatic, calcium messenger system, contact angle i.e. hydrophobicity measurement and finally particle internalization and Phagocytic index. result were exciting with herbal formulation since it was found to effective in correction of cell parameters related to phagocytosis and remarkable recovery in antioxidant enzymes in diabetic patients. Conclusion: In summary, the results obtained in the present investigation demonstrated\ that the present formulation beneficial in management of diabetic complication.