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1.
Article in English | LILACS, BBO | ID: biblio-1056852

ABSTRACT

Abstract Objective: To evaluate the knowledge of elementary school teachers about the management of dental trauma. Material and Methods: An observational study, with the cross-sectional design, was conducted among primary school teachers in Hail, Saudi Arabia during January 2017. The questionnaire distributed among 400 primary school teachers from 18 different schools using convenient sampling. Data were gathered and analyzed using SPSS version 20 Results: 378 (94.5%) respondents to the questionnaire. It was found that only 37.8% of the primary school teachers were able to distinguish between the primary and permanent teeth. Only 59.5% reported starting the management of a child with trauma immediately. Merely 38.4% believed that it is important to search for the missing tooth or the broken pieces, whereas 31% would re-implant the permanent tooth into the socket by themselves. Regarding the storage media, only 16.6% respond correctly. According to school teachers, the best way of learning the management of dental trauma at school is through videos (36.2%) and phone application (33.9%) Conclusion: School teachers lack knowledge regarding the management of dental trauma. We strongly recommend planning for dental trauma educational based on the teacher's perception after pilot testing its effectiveness.


Subject(s)
Saudi Arabia/epidemiology , Tooth Replantation/instrumentation , Health Knowledge, Attitudes, Practice , Tooth Injuries/diagnostic imaging , School Teachers , Surveys and Questionnaires , Data Interpretation, Statistical
2.
Indian J Exp Biol ; 2006 Feb; 44(2): 151-6
Article in English | IMSEAR | ID: sea-59974

ABSTRACT

Optimization of the fermentation medium for maximum alkaline protease production was carried out with a new strain of Pseudomonas aeruginosa (B-2). Replacing the protein source/inducer (albumin in place of casein) brought about significant increase in yield after 48 hr of inoculation. Three most effective medium constituents identified by initial screening method of Plackett-Burman were albumin, (NH4)2SO4 and glucose. Central Composite Design (CCD) and Response Surface Methodology (RSM) were used in the design of the experiment and in the analysis of the results. Optimum levels of the effective medium constituents were albumin (6.586%); (NH4)2SO4, 0.164%; and glucose, 6.72%. The alkaline protease production increased from 533460 to 793492 Ul(-1).


Subject(s)
Ammonium Sulfate/chemistry , Bacterial Proteins/biosynthesis , Bacteriological Techniques , Caseins/chemistry , Cell Culture Techniques , Culture Media/chemistry , Endopeptidases/biosynthesis , Fermentation , Glucose/chemistry , Models, Statistical , Pseudomonas aeruginosa/drug effects , Serum Albumin/chemistry , Time Factors
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